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Molecular and Cellular Biology, October 2004, p. 8600-8610, Vol. 24, No. 19
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.19.8600-8610.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Pre-rRNA Base Pairing and 80S Complex Formation in Subnucleolar Localization of the U3 snoRNP

Sander Granneman,1,{dagger} Judith Vogelzangs,1 Reinhard Lührmann,2 Walther J. van Venrooij,1 Ger J. M. Pruijn,1 and Nicholas J. Watkins2*

Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands,1 Max Planck Institute of Biophysical Chemistry, Göttingen, Germany2

Received 8 March 2004/ Returned for modification 7 April 2004/ Accepted 8 July 2004

In the nucleolus the U3 snoRNA is recruited to the 80S pre-rRNA processing complex in the dense fibrillar component (DFC). The U3 snoRNA is found throughout the nucleolus and has been proposed to move with the preribosomes to the granular component (GC). In contrast, the localization of other RNAs, such as the U8 snoRNA, is restricted to the DFC. Here we show that the incorporation of the U3 snoRNA into the 80S processing complex is not dependent on pre-rRNA base pairing sequences but requires the B/C motif, a U3-specific protein-binding element. We also show that the binding of Mpp10 to the 80S U3 complex is dependent on sequences within the U3 snoRNA that base pair with the pre-rRNA adjacent to the initial cleavage site. Furthermore, mutations that inhibit 80S complex formation and/or the association of Mpp10 result in retention of the U3 snoRNA in the DFC. From this we propose that the GC localization of the U3 snoRNA is a direct result of its active involvement in the initial steps of ribosome biogenesis.

* Corresponding author. Present address: Institute of Cell and Molecular Biosciences, University of Newcastle, The Medical School, Framlington Pl., Newcastle upon Tyne, NE2 4HH, United Kingdom. Phone: 44 191 222 6991. Fax: 44 191 222 7424. E-mail: n.j.watkins{at}ncl.ac.uk.

{dagger} Present address: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Conn.

Molecular and Cellular Biology, October 2004, p. 8600-8610, Vol. 24, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.19.8600-8610.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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