This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yarragudi, A.
Right arrow Articles by Morse, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yarragudi, A.
Right arrow Articles by Morse, R. H.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2004, p. 9152-9164, Vol. 24, No. 20
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.20.9152-9164.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of ABF1 and RAP1 in Chromatin Opening and Transactivator Potentiation in the Budding Yeast Saccharomyces cerevisiae

Arunadevi Yarragudi,1 Tsuyoshi Miyake,2 Rong Li,2 and Randall H. Morse1,3*

Wadsworth Center, New York State Department of Health,1 Department of Biomedical Sciences, University at Albany School of Public Health, Albany, New York,3 Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, Virginia2

Received 22 June 2004/ Returned for modification 1 July 2004/ Accepted 28 July 2004

Autonomously replicating sequence binding factor 1 (ABF1) and repressor/activator protein 1 (RAP1) from budding yeast are multifunctional, site-specific DNA-binding proteins, with roles in gene activation and repression, replication, and telomere structure and function. Previously we have shown that RAP1 can prevent nucleosome positioning in the vicinity of its binding site and have provided evidence that this ability to create a local region of "open" chromatin contributes to RAP1 function at the HIS4 promoter by facilitating binding and activation by GCN4. Here we examine and directly compare to that of RAP1 the ability of ABF1 to create a region of open chromatin near its binding site and to contribute to activated transcription at the HIS4, ADE5,7, and HIS7 promoters. ABF1 behaves similarly to RAP1 in these assays, but it shows some subtle differences from RAP1 in the character of the open chromatin region near its binding site. Furthermore, although the two factors can similarly enhance activated transcription at the promoters tested, RAP1 binding is continuously required for this enhancement, but ABF1 binding is not. These results indicate that ABF1 and RAP1 achieve functional similarity in part via mechanistically distinct pathways.

* Corresponding author. Mailing address: Wadsworth Center, Albany, NY 12201-2002. Phone: (518) 486-3116. Fax: (518) 474-3181. E-mail: randall.morse{at}wadsworth.org.

Molecular and Cellular Biology, October 2004, p. 9152-9164, Vol. 24, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.20.9152-9164.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gordan, R., Hartemink, A. J., Bulyk, M. L. (2009). Distinguishing direct versus indirect transcription factor-DNA interactions. Genome Res 19: 2090-2100 [Abstract] [Full Text]  
  • Swamy, K. B. S., Cho, C.-Y., Chiang, S., Tsai, Z. T.-Y., Tsai, H.-K. (2009). Impact of DNA-binding position variants on yeast gene expression. Nucleic Acids Res 0: gkp743v1-gkp743 [Abstract] [Full Text]  
  • Yu, S., Smirnova, J. B., Friedberg, E. C., Stillman, B., Akiyama, M., Owen-Hughes, T., Waters, R., Reed, S. H. (2009). ABF1-binding Sites Promote Efficient Global Genome Nucleotide Excision Repair. J. Biol. Chem. 284: 966-973 [Abstract] [Full Text]  
  • Schlecht, U., Erb, I., Demougin, P., Robine, N., Borde, V., Nimwegen, E. v., Nicolas, A., Primig, M. (2008). Genome-wide Expression Profiling, In Vivo DNA Binding Analysis, and Probabilistic Motif Prediction Reveal Novel Abf1 Target Genes during Fermentation, Respiration, and Sporulation in Yeast. Mol. Biol. Cell 19: 2193-2207 [Abstract] [Full Text]  
  • Kasahara, K., Ohtsuki, K., Ki, S., Aoyama, K., Takahashi, H., Kobayashi, T., Shirahige, K., Kokubo, T. (2007). Assembly of Regulatory Factors on rRNA and Ribosomal Protein Genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 6686-6705 [Abstract] [Full Text]  
  • Yarragudi, A., Parfrey, L. W., Morse, R. H. (2007). Genome-wide analysis of transcriptional dependence and probable target sites for Abf1 and Rap1 in Saccharomyces cerevisiae. Nucleic Acids Res 35: 193-202 [Abstract] [Full Text]  
  • Zou, Y., Yu, Q., Bi, X. (2006). Asymmetric Positioning of Nucleosomes and Directional Establishment of Transcriptionally Silent Chromatin by Saccharomyces cerevisiae Silencers. Mol. Cell. Biol. 26: 7806-7819 [Abstract] [Full Text]  
  • Zou, Y., Yu, Q., Chiu, Y.-H., Bi, X. (2006). Position Effect on the Directionality of Silencer Function in Saccharomyces cerevisiae. Genetics 174: 203-213 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»