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Molecular and Cellular Biology, November 2004, p. 9823-9834, Vol. 24, No. 22
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.22.9823-9834.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Architectural Role of Mitochondrial Transcription Factor A in Maintenance of Human Mitochondrial DNA

Tomotake Kanki,1 Kippei Ohgaki,1 Martina Gaspari,2 Claes M. Gustafsson,2 Atsushi Fukuoh,1 Narie Sasaki,3 Naotaka Hamasaki,1 and Dongchon Kang1*

Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, Higashi-ku, Fukuoka,1 Department of Biology, Faculty of Science, Ochanomizu University, Tokyo, Japan,3 Department of Medical Nutrition, Karolinska Institute, Novum, Hudddinge, Sweden2

Received 29 December 2003/ Returned for modification 17 March 2004/ Accepted 26 August 2004

Mitochondrial transcription factor A (TFAM), a transcription factor for mitochondrial DNA (mtDNA) that also possesses the property of nonspecific DNA binding, is essential for maintenance of mtDNA. To clarify the role of TFAM, we repressed the expression of endogenous TFAM in HeLa cells by RNA interference. The amount of TFAM decreased maximally to about 15% of the normal level at day 3 after RNA interference and then recovered gradually. The amount of mtDNA changed closely in parallel with the daily change in TFAM while in organello transcription of mtDNA at day 3 was maintained at about 50% of the normal level. TFAM lacking its C-terminal 25 amino acids (TFAM-{Delta}C) marginally activated transcription in vitro. When TFAM-{Delta}C was expressed at levels comparable to those of endogenous TFAM in HeLa cells, mtDNA increased twofold, suggesting that TFAM-{Delta}C is as competent in maintaining mtDNA as endogenous TFAM under these conditions. The in organello transcription of TFAM-{Delta}C-expressing cells was no more than that in the control. Thus, the mtDNA amount is finely correlated with the amount of TFAM but not with the transcription level. We discuss an architectural role for TFAM in the maintenance of mtDNA in addition to its role in transcription activation.

* Corresponding author. Mailing address: Department of Clinical Chemistry and Laboratory Medicine, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-5749. Fax: 81-92-642-5772. E-mail: kang{at}mailserver.med.kyushu-u.ac.jp.

Molecular and Cellular Biology, November 2004, p. 9823-9834, Vol. 24, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.22.9823-9834.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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