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Molecular and Cellular Biology, November 2004, p. 9873-9886, Vol. 24, No. 22
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.22.9873-9886.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Functional Dissection of the Localization-Specific Roles of Budding Yeast Polo Kinase Cdc5p

Jung-Eun Park,1,2 Chong J. Park,1 Krisada Sakchaisri,1,3 Tatiana Karpova,4 Satoshi Asano,1 James McNally,4 Yangil Sunwoo,2 Sun-Hee Leem,2 and Kyung S. Lee1*

Laboratory of Metabolism,1 Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland,4 Department of Biology, Dong-A University, Busan, Korea,2 Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand3

Received 18 May 2004/ Accepted 30 July 2004

Budding yeast polo kinase Cdc5p localizes to the spindle pole body (SPB) and to the bud-neck and plays multiple roles during M-phase progression. To dissect localization-specific mitotic functions of Cdc5p, we tethered a localization-defective N-terminal kinase domain of Cdc5p (Cdc5p{Delta}C) to the SPB or to the bud-neck with components specifically localizing to one of these sites and characterized these mutants in a cdc5{Delta} background. Characterization of a viable, SPB-localizing, CDC5{Delta}C-CNM67 mutant revealed that it is defective in timely degradation of Swe1p, a negative regulator of Cdc28p. Loss of BFA1, a negative regulator of mitotic exit, rescued the lethality of a neck-localizing CDC5{Delta}C-CDC12 or CDC5{Delta}C-CDC3 mutant but yielded severe defects in cytokinesis. These data suggest that the SPB-associated Cdc5p activity is critical for both mitotic exit and cytokinesis, whereas the bud neck-localized Cdc5p is required for proper Swe1p regulation. Interestingly, a cdc5{Delta} bfa1{Delta} swe1{Delta} triple mutant is viable but grows slowly, whereas cdc5{Delta} cells bearing both CDC5{Delta}C-CNM67 and CDC5{Delta}C-CDC12 grow well with only a mild cell cycle delay. Thus, SPB- and the bud-neck-localized Cdc5p control most of the critical Cdc5p functions and downregulation of Bfa1p and Swe1p at the respective locations are two critical factors that require Cdc5p.

* Corresponding author. Mailing address: Laboratory of Metabolism, National Cancer Institute, NIH, 9000 Rockville Pike, Bldg. 37, Rm. 3118, Bethesda, MD 20892. Phone: (301) 496-9635. Fax: (301) 496-8419. E-mail: kyunglee{at}pop.nci.nih.gov.

Molecular and Cellular Biology, November 2004, p. 9873-9886, Vol. 24, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.22.9873-9886.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Liang, F., Jin, F., Liu, H., Wang, Y. (2009). The Molecular Function of the Yeast Polo-like Kinase Cdc5 in Cdc14 Release during Early Anaphase. Mol. Biol. Cell 20: 3671-3679 [Abstract] [Full Text]  
  • Archambault, V., D'Avino, P. P., Deery, M. J., Lilley, K. S., Glover, D. M. (2008). Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosis. Genes Dev. 22: 2707-2720 [Abstract] [Full Text]  
  • Park, C. J., Park, J.-E., Karpova, T. S., Soung, N.-K., Yu, L.-R., Song, S., Lee, K. H., Xia, X., Kang, E., Dabanoglu, I., Oh, D.-Y., Zhang, J. Y., Kang, Y. H., Wincovitch, S., Huffaker, T. C., Veenstra, T. D., McNally, J. G., Lee, K. S. (2008). Requirement for the Budding Yeast Polo Kinase Cdc5 in Proper Microtubule Growth and Dynamics. Eukaryot Cell 7: 444-453 [Abstract] [Full Text]  


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