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Molecular and Cellular Biology, April 2004, p. 2605-2613, Vol. 24, No. 7
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.7.2605-2613.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Histone Fold Protein Dls1p Is Required for Isw2-Dependent Chromatin Remodeling In Vivo

Audrey D. McConnell,1 Marnie E. Gelbart,1,2 and Toshio Tsukiyama1*

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,1 Molecular and Cellular Biology Program, Fred Hutchinson Cancer Research Center and University of Washington, Seattle, Washington 981952

Received 9 October 2003/ Returned for modification 9 November 2003/ Accepted 11 January 2004

We report the identification of two new subunits of the Isw2 chromatin-remodeling complex in Saccharomyces cerevisiae. Both proteins, Dpb4p and Yjl065cp (named Dls1p), contain histone fold motifs and are homologous to the two smallest subunits of ISWI-containing CHRAC complexes in higher eukaryotes. Dpb4p is also a subunit of the DNA polymerase epsilon (pol{varepsilon}) complex, and Dls1p is homologous to another pol{varepsilon} subunit, Dpb3p. Therefore, these small histone fold proteins may fulfill functions that are required for both pol{varepsilon} and Isw2 complexes. We characterized the role of Dls1p in known roles of the Isw2 complex in vivo. Transcriptional analyses reveal that the Isw2 complex requires Dls1p to various degrees at a wide variety of loci in vivo. Consistent with this, Dls1p is required for Isw2-dependent chromatin remodeling in vivo, although the requirement for this protein varies among Isw2 targets. Dls1p is likely required for functions of the Isw2 complex at steps subsequent to its interaction with chromatin, since a dls1 mutation does not affect cross-linking of Isw2 with chromatin.

* Corresponding author. Mailing address: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Mail Stop A1-162, 1100 Fairview Ave. North, Seattle, WA 98109-1024. Phone: (206) 667-4996. Fax: (206) 667-6497. E-mail: ttsukiya{at}fhcrc.org.

Molecular and Cellular Biology, April 2004, p. 2605-2613, Vol. 24, No. 7
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.7.2605-2613.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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