This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kuan, Y.-S.
Right arrow Articles by Searles, L. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuan, Y.-S.
Right arrow Articles by Searles, L. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2004, p. 3734-3746, Vol. 24, No. 9
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.9.3734-3746.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Suppressor of sable, a Putative RNA-Processing Protein, Functions at the Level of Transcription

Yung-Shu Kuan,{dagger} Paul Brewer-Jensen, and Lillie L. Searles*

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-3280

Received 28 August 2003/ Returned for modification 13 October 2003/ Accepted 30 January 2004

The Drosophila melanogaster su(s) gene product negatively regulates the expression of mutant alleles with transposon insertions in the 5'-transcribed region by an unknown mechanism. We have investigated here su(s) function through in vivo structure-function analysis, heterologous reporter gene assays, and in vivo transcriptional induction experiments. We have shown that mutations of two arginine-rich motifs (ARMs), an acidic region, or two CCCH zinc fingers affect the ability of Su(s) to downregulate the expression of an insertion mutant allele and to autoregulate genomic su(s) transgenes. Using yeast and HeLa cell assays, we found that, when tethered to the promoter region, the N- and C-terminal regions of Su(s) can repress reporter gene expression, and all three motifs, but most significantly the ARMs, contribute to the repression activity. Finally, we showed that, in vivo, Su(s) inhibits the transcriptional induction of a transgene with an insertion in the first exon but does not affect induction of a similar transgene with a consensus 5' splice site near the upstream boundary of the insertion. Together, these results reveal a link between Su(s), transcription, and pre-mRNA processing.

* Corresponding author. Mailing address: Department of Biology, CB#3280, UNC at Chapel Hill, Chapel Hill, NC 27599-3280. Phone: (919) 966-4989. Fax: (919) 962-1625. E-mail: lsearles{at}email.unc.edu.

{dagger} Present address: Department of Embryology, Carnegie Institution, Baltimore, Md.

Molecular and Cellular Biology, May 2004, p. 3734-3746, Vol. 24, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.9.3734-3746.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kuan, Y.-S., Brewer-Jensen, P., Bai, W.-L., Hunter, C., Wilson, C. B., Bass, S., Abernethy, J., Wing, J. S., Searles, L. L. (2009). Drosophila Suppressor of Sable Protein [Su(s)] Promotes Degradation of Aberrant and Transposon-Derived RNAs. Mol. Cell. Biol. 29: 5590-5603 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»