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Molecular and Cellular Biology, May 2004, p. 4049-4064, Vol. 24, No. 9
0270-7306/04/$08.00+0     DOI: 10.1128/MCB.24.9.4049-4064.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Saccharomyces cerevisiae Flap Endonuclease 1 Uses Flap Equilibration To Maintain Triplet Repeat Stability

Yuan Liu,1,{dagger} Haihua Zhang,2,{dagger} Janaki Veeraraghavan,1 Robert A. Bambara,1 and Catherine H. Freudenreich2,3*

Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642,1 Department of Biology,2 Program in Genetics, Tufts University, Medford, Massachusetts 021553

Received 13 November 2003/ Returned for modification 22 December 2003/ Accepted 9 February 2004

Flap endonuclease 1 (FEN1) is a central component of Okazaki fragment maturation in eukaryotes. Genetic analysis of Saccharomyces cerevisiae FEN1 (RAD27) also reveals its important role in preventing trinucleotide repeat (TNR) expansion. In humans such expansion is associated with neurodegenerative diseases. In vitro, FEN1 can inhibit TNR expansion by employing its endonuclease activity to compete with DNA ligase I. Here we employed two yeast FEN1 nuclease mutants, rad27-G67S and rad27-G240D, to further define the mechanism by which FEN1 prevents TNR expansion. Using a yeast artificial chromosome system that can detect both TNR instability and fragility, we demonstrate that the G240D but not the G67S mutation increases both the expansion and fragility of a CTG tract in vivo. In vitro, the G240D nuclease is proficient in cleaving a fixed nonrepeat double flap; however, it exhibits severely impaired cleavage of both nonrepeat and CTG-containing equilibrating flaps. In contrast, wild-type FEN1 and the G67S mutant exhibit more efficient cleavage on an equilibrating flap than on a fixed CTG flap. The degree of TNR expansion and the amount of chromosome fragility observed in the mutant strains correlate with the severity of defective flap cleavage in vitro. We present a model to explain how flap equilibration and the unique tracking mechanism of FEN1 can collaborate to remove TNR flaps and prevent repeat expansion.

* Corresponding author. Mailing address: Department of Biology, Tufts University, 165 Packard, Medford, MA 02155. Phone: (617) 627-4037. Fax: (617) 627-3805. E-mail: catherine.freudenreich{at}tufts.edu.

{dagger} Y.L. and H.Z. contributed equally to this work.

Molecular and Cellular Biology, May 2004, p. 4049-4064, Vol. 24, No. 9
0022-538X/04/$08.00+0     DOI: 10.1128/MCB.24.9.4049-4064.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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