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Molecular and Cellular Biology, June 2005, p. 5084-5094, Vol. 25, No. 12
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.12.5084-5094.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Oct-3/4 Maintains the Proliferative Embryonic Stem Cell State via Specific Binding to a Variant Octamer Sequence in the Regulatory Region of the UTF1 Locus

Masazumi Nishimoto,1 Satoru Miyagi,1,2 Toshiyuki Yamagishi,3 Takehisa Sakaguchi,1 Hitoshi Niwa,4 Masami Muramatsu,1 and Akihiko Okuda1*

Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan,1 REDS Group, Saitama Small Enterprise Promotion Corporation, Skip City, Kawaguchi, Saitama 333-0844, Japan,2 Department of Anatomy, Saitama Medical School, 38 Morohongo Moroyama Iruma-gun, Saitama 350-0495, Japan,3 Laboratory for Pluripotent Cell Studies, RIKEN, Center for Developmental Biology, 2-2-3 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan4

Received 2 February 2005/ Returned for modification 2 March 2005/ Accepted 11 March 2005

The POU transcription factor Oct-3/4 has been shown to be critical for maintaining embryonic stem (ES) cell character. However, the molecular mechanisms underlying its function remain elusive. We have previously shown that among the POU transcription factor family of proteins, Oct-3/4 alone is able to bind to the regulatory region of the UTF1 gene bearing a variant octamer sequence together with Sox-2. Here, we demonstrate using Oct-3/4-Oct-6 chimeras that there is a precise correlation between the ability of proteins to form a complex on the UTF1 enhancer with Sox-2 and the ability to maintain the stem cell state in ES cells. Different chimeric proteins show differential abilities to form a Sox-2-containing complex on the UTF1 regulatory region, with a decrease in efficiency of the complex formation accompanied by a decrease in the level of UTF1 expression and the rate of cell proliferation. Overexpression of UTF1 in these slow-growing cells was able to restore their proliferation rate to wild-type levels. Moreover, UTF1 was also observed to have an effect on teratoma formation. These results suggest a molecular pathway by which Oct-3/4 induces rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene.

* Corresponding author. Mailing address: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical School, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan. Phone: 81-42-985-7268. Fax: 81-42-985-7264. E-mail: akiokuda{at}saitama-med.ac.jp.

Molecular and Cellular Biology, June 2005, p. 5084-5094, Vol. 25, No. 12
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.12.5084-5094.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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