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Molecular and Cellular Biology, August 2005, p. 6330-6337, Vol. 25, No. 15
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.15.6330-6337.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Meiosis-Specific Cyclin Regulated by Splicing Is Required for Proper Progression through Meiosis

Jordi Malapeira,¹ Alberto Moldón,¹ Elena Hidalgo,¹ Gerald R. Smith,² Paul Nurse,^3* and José Ayté^1*

Cell Signaling Unit, Universitat Pompeu Fabra, Barcelona, Spain,¹ Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,² The Rockefeller University, New York, New York 10021³

Received 6 April 2005/ Returned for modification 4 May 2005/ Accepted 13 May 2005

The meiotic cell cycle is modified from the mitotic cell cycle by having a premeiotic S phase which leads to high levels of recombination, a reductional pattern of chromosome segregation at the first division, and a second division with no intervening DNA synthesis. Cyclin-dependent kinases are essential for progression through the meiotic cell cycle, as for the mitotic cycle. Here we show that a fission yeast cyclin, Rem1, is present only during meiosis. Cells lacking Rem1 have impaired meiotic recombination, and Rem1 is required for premeiotic DNA synthesis when Cig2 is not present. rem1 expression is regulated at the level of both transcription and splicing, with Mei4 as a positive and Cig2 a negative factor of rem1 splicing. This regulation ensures the timely appearance of the different cyclins during meiosis, which is required for the proper progression through the meiotic cell cycle. We propose that the meiosis-specific B-type cyclin Rem1 has a central role in bringing about progression through meiosis.

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* Corresponding author. Mailing address for Paul Nurse: The Rockefeller University, 1230 York Avenue, New York, NY 10021. Phone: (212) 327 8080. Fax: (212) 327 8900. E-mail: nurse{at}rockefeller.edu. Mailing address for José Ayté: Cell Signalling Unit, Universitat Pompeu Fabra, c/ Doctor Aiguader 80, Barcelona 08003, Spain. Phone: 34 93 542 2891. Fax: 34 93 542 2802. E-mail: jose.ayte{at}upf.edu.

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Molecular and Cellular Biology, August 2005, p. 6330-6337, Vol. 25, No. 15
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.15.6330-6337.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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