This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Swaminathan, V.
Right arrow Articles by Kundu, T. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Swaminathan, V.
Right arrow Articles by Kundu, T. K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2005, p. 7534-7545, Vol. 25, No. 17
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.17.7534-7545.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Human Histone Chaperone Nucleophosmin Enhances Acetylation-Dependent Chromatin Transcription{dagger}

V. Swaminathan, A. Hari Kishore, K. K. Febitha, and Tapas K. Kundu*

Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur, Bangalore 64, India

Received 1 February 2005/ Returned for modification 23 February 2005/ Accepted 23 May 2005

Histone chaperones are a group of proteins that aid in the dynamic chromatin organization during different cellular processes. Here, we report that the human histone chaperone nucleophosmin interacts with the core histones H3, H2B, and H4 but that this histone interaction is not sufficient to confer the chaperone activity. Significantly, nucleophosmin enhances the acetylation-dependent chromatin transcription and it becomes acetylated both in vitro and in vivo. Acetylation of nucleophosmin and the core histones was found to be essential for the enhancement of chromatin transcription. The acetylated NPM1 not only shows an increased affinity toward acetylated histones but also shows enhanced histone transfer ability. Presumably, nucleophosmin disrupts the nucleosomal structure in an acetylation-dependent manner, resulting in the transcriptional activation. These results establish nucleophosmin (NPM1) as a human histone chaperone that becomes acetylated, resulting in the enhancement of chromatin transcription.

* Corresponding author. Mailing address: Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur, Bangalore 64, India. Phone: 91-80-22082840. Fax: 91-80-22082766. E-mail: tapas{at}jncasr.ac.in.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, September 2005, p. 7534-7545, Vol. 25, No. 17
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.17.7534-7545.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pradeepa, M. M., Nikhil, G., Hari Kishore, A., Bharath, G. N., Kundu, T. K., Rao, M. R. S. (2009). Acetylation of Transition Protein 2 (TP2) by KAT3B (p300) Alters Its DNA Condensation Property and Interaction with Putative Histone Chaperone NPM3. J. Biol. Chem. 284: 29956-29967 [Abstract] [Full Text]  
  • Shandilya, J., Swaminathan, V., Gadad, S. S., Choudhari, R., Kodaganur, G. S., Kundu, T. K. (2009). Acetylated NPM1 Localizes in the Nucleoplasm and Regulates Transcriptional Activation of Genes Implicated in Oral Cancer Manifestation. Mol. Cell. Biol. 29: 5115-5127 [Abstract] [Full Text]  
  • Vascotto, C., Fantini, D., Romanello, M., Cesaratto, L., Deganuto, M., Leonardi, A., Radicella, J. P., Kelley, M. R., D'Ambrosio, C., Scaloni, A., Quadrifoglio, F., Tell, G. (2009). APE1/Ref-1 Interacts with NPM1 within Nucleoli and Plays a Role in the rRNA Quality Control Process. Mol. Cell. Biol. 29: 1834-1854 [Abstract] [Full Text]  
  • Csiszar, A., Wang, M., Lakatta, E. G., Ungvari, Z. (2008). Inflammation and endothelial dysfunction during aging: role of NF-{kappa}B. J. Appl. Physiol. 105: 1333-1341 [Abstract] [Full Text]  
  • Lindstrom, M. S., Zhang, Y. (2008). Ribosomal Protein S9 Is a Novel B23/NPM-binding Protein Required for Normal Cell Proliferation. J. Biol. Chem. 283: 15568-15576 [Abstract] [Full Text]  
  • Murano, K., Okuwaki, M., Hisaoka, M., Nagata, K. (2008). Transcription Regulation of the rRNA Gene by a Multifunctional Nucleolar Protein, B23/Nucleophosmin, through Its Histone Chaperone Activity. Mol. Cell. Biol. 28: 3114-3126 [Abstract] [Full Text]  
  • Okuwaki, M. (2008). The Structure and Functions of NPM1/Nucleophsmin/B23, a Multifunctional Nucleolar Acidic Protein. J Biochem 143: 441-448 [Abstract] [Full Text]  
  • Zou, Y., Wu, J., Giannone, R. J., Boucher, L., Du, H., Huang, Y., Johnson, D. K., Liu, Y., Wang, Y. (2008). Nucleophosmin/B23 Negatively Regulates GCN5-dependent Histone Acetylation and Transactivation. J. Biol. Chem. 283: 5728-5737 [Abstract] [Full Text]  
  • Ganapathi, K. A., Austin, K. M., Lee, C.-S., Dias, A., Malsch, M. M., Reed, R., Shimamura, A. (2007). The human Shwachman-Diamond syndrome protein, SBDS, associates with ribosomal RNA. Blood 110: 1458-1465 [Abstract] [Full Text]  
  • Xu, Y., Fang, F., Dhar, S. K., St. Clair, W. H., Kasarskis, E. J., St. Clair, D. K. (2007). The Role of a Single-stranded Nucleotide Loop in Transcriptional Regulation of the Human sod2 Gene. J. Biol. Chem. 282: 15981-15994 [Abstract] [Full Text]  
  • Falini, B., Nicoletti, I., Bolli, N., Martelli, M. P., Liso, A., Gorello, P., Mandelli, F., Mecucci, C., Martelli, M. F. (2007). Translocations and mutations involving the nucleophosmin (NPM1) gene in lymphomas and leukemias. haematol 92: 519-532 [Abstract] [Full Text]  
  • Rickards, B., Flint, S. J., Cole, M. D., LeRoy, G. (2007). Nucleolin Is Required for RNA Polymerase I Transcription In Vivo. Mol. Cell. Biol. 27: 937-948 [Abstract] [Full Text]  
  • Workman, J. L. (2006). Nucleosome displacement in transcription. Genes Dev. 20: 2009-2017 [Abstract] [Full Text]  
  • Eirin-Lopez, J. M., Frehlick, L. J., Ausio, J. (2006). Long-Term Evolution and Functional Diversification in the Members of the Nucleophosmin/Nucleoplasmin Family of Nuclear Chaperones. Genetics 173: 1835-1850 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»