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Molecular and Cellular Biology, September 2005, p. 7616-7624, Vol. 25, No. 17
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.17.7616-7624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Structural Characterization of the Histone Variant macroH2A

Srinivas Chakravarthy,1 Sampath Kumar Y. Gundimella,1 Cecile Caron,2 Pierre-Yves Perche,2 John R. Pehrson,3 Saadi Khochbin,2 and Karolin Luger1*

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, Colorado 80523-1870,1 INSERM U309-Laboratoire de Biologie Moléculaire et Cellulaire de la Différenciation-Équipe Chromatine et Expression des genes, Institut Albert Bonniot, Faculté de médecine, Domaine de la Merci, 38 706 La Tronche, France,2 Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 191043

Received 23 March 2005/ Returned for modification 15 April 2005/ Accepted 6 June 2005

macroH2A is an H2A variant with a highly unusual structural organization. It has a C-terminal domain connected to the N-terminal histone domain by a linker. Crystallographic and biochemical studies show that changes in the L1 loop in the histone fold region of macroH2A impact the structure and potentially the function of nucleosomes. The 1.6-Å X-ray structure of the nonhistone region reveals an {alpha}/ß fold which has previously been found in a functionally diverse group of proteins. This region associates with histone deacetylases and affects the acetylation status of nucleosomes containing macroH2A. Thus, the unusual domain structure of macroH2A integrates independent functions that are instrumental in establishing a structurally and functionally unique chromatin domain.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870. Phone: (970) 491-6405. Fax: (970) 491-0494. E-mail: kluger{at}lamar.colostate.edu.

Molecular and Cellular Biology, September 2005, p. 7616-7624, Vol. 25, No. 17
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.17.7616-7624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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