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Molecular and Cellular Biology, November 2005, p. 9283-9291, Vol. 25, No. 21
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.21.9283-9291.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Pairing between gypsy Insulators Facilitates the Enhancer Action in trans throughout the Drosophila Genome

Elena Kravchenko,1 Ekaterina Savitskaya,1,2 Oksana Kravchuk,1 Alexander Parshikov,1 Pavel Georgiev,1* and Mikhail Savitsky1,2*

Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334,1 Center for Medical Studies of Oslo University, Moscow 199334, Russia2

Received 20 September 2004/ Returned for modification 17 November 2004/ Accepted 25 July 2005

The Suppressor of the Hairy wing [Su(Hw)] binding region within the gypsy retrotransposon is the best known chromatin insulator in Drosophila melanogaster. According to previous data, two copies of the gypsy insulator inserted between an enhancer and a promoter neutralize each other's actions, which is indicative of an interaction between the protein complexes bound to the insulators. We have investigated the role of pairing between the gypsy insulators located on homologous chromosomes in trans interaction between yellow enhancers and a promoter. It has been shown that trans activation of the yellow promoter strongly depends on the site of the transposon insertion, which is evidence for a role of surrounding chromatin in homologous pairing. The presence of the gypsy insulators in both homologous chromosomes even at a distance of 9 kb downstream from the promoter dramatically improves the trans activation of yellow. Moreover, the gypsy insulators have proved to stabilize trans activation between distantly located enhancers and a promoter. These data suggest that gypsy insulator pairing is involved in communication between loci in the Drosophila genome.

* Corresponding author. Mailing address: Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., 119334 Moscow, Russia. Phone: 7-095-1354109. Fax: 7-095-1354105. E-mail for Mikhail Savitsky: msavitsky{at}newmail.ru. E-mail for Pavel Georgiev: georgiev_p{at}mail.ru.

Molecular and Cellular Biology, November 2005, p. 9283-9291, Vol. 25, No. 21
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.21.9283-9291.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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