This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Edlund, S.
Right arrow Articles by Landström, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edlund, S.
Right arrow Articles by Landström, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2005, p. 1475-1488, Vol. 25, No. 4
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.4.1475-1488.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Interaction between Smad7 and ß-Catenin: Importance for Transforming Growth Factor ß-Induced Apoptosis{dagger}

Sofia Edlund,1 So Young Lee,1,{ddagger} Susanne Grimsby,1 Shouthing Zhang,1 Pontus Aspenström,1 Carl-Henrik Heldin,1 and Maréne Landström1*

Ludwig Institute for Cancer Research, Uppsala, Sweden1

Received 9 July 2004/ Returned for modification 24 August 2004/ Accepted 29 October 2004

Members of the transforming growth factor ß (TGF-ß) and Wnt/wingless superfamilies regulate cell fate during development and tissue maintenance. Here we report that Smad7 interacts with ß-catenin and lymphoid enhancer binding factor 1/T-cell-specific factor (LEF1/TCF), transcriptional regulators in Wnt signaling, in a TGF-ß-dependent manner. Smad7 was found to be required for TGF-ß1-induced accumulation of ß-catenin and LEF1 in human prostate cancer (PC-3U) cells as well as in human keratinocytes (HaCaT cells). Moreover, when the endogenous Smad7 was repressed by specific small interfering RNA, TGF-ß-induced increase of activated p38, Akt phosphorylated on Ser473, glycogen synthase kinase 3ß phosphorylated on Ser9 was prevented, as well as the TGF-ß-induced association between ß-catenin and LEF1. Notably, the observed physical association of Smad7 and ß-catenin was found to be important for TGF-ß-induced apoptosis, since suppression of ß-catenin expression by small interfering RNA decreased the apoptotic response to TGF-ß.

* Corresponding author. Mailing address: Ludwig Institute for Cancer Research, Box 595, Biomedical Center, SE 751 24 Uppsala, Sweden. Phone: 46-18-16 04 06. Fax: 46-18-16 04 20. E-mail: Marene.Landstrom{at}LICR.uu.se.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, February 2005, p. 1475-1488, Vol. 25, No. 4
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.4.1475-1488.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dennler, S., Andre, J., Verrecchia, F., Mauviel, A. (2009). Cloning of the Human GLI2 Promoter: TRANSCRIPTIONAL ACTIVATION BY TRANSFORMING GROWTH FACTOR-{beta} VIA SMAD3/{beta}-CATENIN COOPERATION. J. Biol. Chem. 284: 31523-31531 [Abstract] [Full Text]  
  • Mauro, T. M., McCormick, J. A., Wang, J., Boini, K. M., Ray, L., Monks, B., Birnbaum, M. J., Lang, F., Pearce, D. (2009). Akt2 and SGK3 are both determinants of postnatal hair follicle development. FASEB J. 23: 3193-3202 [Abstract] [Full Text]  
  • Yan, X., Liu, Z., Chen, Y. (2009). Regulation of TGF-{beta} signaling by Smad7. Acta Biochim Biophys Sin 41: 263-272 [Abstract] [Full Text]  
  • Tang, Y., Liu, Z., Zhao, L., Clemens, T. L., Cao, X. (2008). Smad7 Stabilizes {beta}-Catenin Binding to E-cadherin Complex and Promotes Cell-Cell Adhesion. J. Biol. Chem. 283: 23956-23963 [Abstract] [Full Text]  
  • Hayward, P., Kalmar, T., Martinez Arias, A. (2008). Wnt/Notch signalling and information processing during development. Development 135: 411-424 [Abstract] [Full Text]  
  • Fosslien, E. (2008). Cancer Morphogenesis: Role of Mitochondrial Failure. Annals of Clinical & Laboratory Science 38: 307-330 [Abstract] [Full Text]  
  • Zhang, S., Fei, T., Zhang, L., Zhang, R., Chen, F., Ning, Y., Han, Y., Feng, X.-H., Meng, A., Chen, Y.-G. (2007). Smad7 Antagonizes Transforming Growth Factor {beta} Signaling in the Nucleus by Interfering with Functional Smad-DNA Complex Formation. Mol. Cell. Biol. 27: 4488-4499 [Abstract] [Full Text]  
  • Zhao, B. M., Hoffmann, F. M. (2006). Inhibition of Transforming Growth Factor-beta1-induced Signaling and Epithelial-to-Mesenchymal Transition by the Smad-binding Peptide Aptamer Trx-SARA. Mol. Biol. Cell 17: 3819-3831 [Abstract] [Full Text]  
  • Yano, T., Ito, K., Fukamachi, H., Chi, X.-Z., Wee, H.-J., Inoue, K.-i., Ida, H., Bouillet, P., Strasser, A., Bae, S.-C., Ito, Y. (2006). The RUNX3 Tumor Suppressor Upregulates Bim in Gastric Epithelial Cells Undergoing Transforming Growth Factor {beta}-Induced Apoptosis. Mol. Cell. Biol. 26: 4474-4488 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»