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Molecular and Cellular Biology, March 2005, p. 1860-1868, Vol. 25, No. 5
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.5.1860-1868.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Early Expression of Yeast Genes Affected by Chemical Stress{dagger}

A. Lucau-Danila,1,{ddagger} G. Lelandais,1,{ddagger} Z. Kozovska,2 V. Tanty,1 T. Delaveau,1 F. Devaux,1 and C. Jacq1*

Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, Paris, France,1 Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic2

Received 6 September 2004/ Returned for modification 8 November 2004/ Accepted 24 November 2004

The variety of environmental stresses is probably the major challenge imposed on transcription activators and the transcriptional machinery. To precisely describe the very early genomic response developed by yeast to accommodate a chemical stress, we performed time course analyses of the modifications of the yeast gene expression program which immediately follows the addition of the antimitotic drug benomyl. Similar analyses were conducted with different isogenic yeast strains in which genes coding for relevant transcription factors were deleted and coupled with efficient bioinformatics tools. Yap1 and Pdr1, two well-known key mediators of stress tolerance, appeared to be responsible for the very rapid establishment of a transient transcriptional response encompassing 119 genes. Yap1, which plays a predominant role in this response, binds, in vivo, promoters of genes which are not automatically up-regulated. We proposed that Yap1 nuclear localization and DNA binding are necessary but not sufficient to elicit the specificity of the chemical stress response.

* Corresponding author. Mailing address: Laboratoire de Génétique Moléculaire, CNRS UMR 8541, Ecole Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex 05, France. Phone: 33 1 44 32 35 46. Fax: 33 1 44 32 35 70. E-mail: jacq{at}biologie.ens.fr.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

{ddagger} A.L.-D. and G.L. contributed equally to this work.

Molecular and Cellular Biology, March 2005, p. 1860-1868, Vol. 25, No. 5
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.5.1860-1868.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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