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Molecular and Cellular Biology, April 2005, p. 2583-2592, Vol. 25, No. 7
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.7.2583-2592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

HCP-4/CENP-C Promotes the Prophase Timing of Centromere Resolution by Enabling the Centromere Association of HCP-6 in Caenorhabditis elegans{dagger}

Landon L. Moore,1* Gerald Stanvitch,1 Mark B. Roth,2 and David Rosen1

Department of Genetics and Genomics, Boston University School of Medicine, Boston, Massachusetts,1 Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington2

Received 15 October 2004/ Returned for modification 15 November 2004/ Accepted 3 January 2005

Prior to microtubule capture, sister centromeres resolve from one another, coming to rest on opposite surfaces of the condensing chromosome. Subsequent assembly of sister kinetochores at each sister centromere generates a geometry favorable for equal levels of segregation of chromatids. The holocentric chromosomes of Caenorhabditis elegans are uniquely suited for the study of centromere resolution and subsequent kinetochore assembly. In C. elegans, only two proteins have been identified as being necessary for centromere resolution, the kinase AIR-2 (prophase only) and the centromere protein HCP-4/CENP-C. Here we found that the loss of proteins involved in chromosome cohesion bypassed the requirement for HCP-4/CENP-C but not for AIR-2. Interestingly, the loss of cohesin proteins also restored the localization of HCP-6 to the kinetochore. The loss of the condensin II protein HCP-6 or MIX-1/SMC2 impaired centromere resolution. Furthermore, the loss of HCP-6 or MIX-1/SMC2 resulted in no centromere resolution when either nocodazole or RNA interference (RNAi) of the kinetochore protein KNL-1 perturbed spindle-kinetochore interactions. This result suggests that normal prophase centromere resolution is mediated by condensin II proteins, which are actively recruited to sister centromeres to mediate the process of resolution.

* Corresponding author. Mailing address: Boston University School of Medicine, 715 Albany St., E642, Boston, MA 02118. Phone: (617) 414-1619. Fax: (617) 414-1646. E-mail: lmoore{at}bu.edu.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, April 2005, p. 2583-2592, Vol. 25, No. 7
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.7.2583-2592.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stanvitch, G., Moore, L. L. (2008). cin-4, a Gene With Homology to Topoisomerase II, Is Required for Centromere Resolution by Cohesin Removal From Sister Kinetochores During Mitosis. Genetics 178: 83-97 [Abstract] [Full Text]  
  • Kwon, M.-S., Hori, T., Okada, M., Fukagawa, T. (2007). CENP-C Is Involved in Chromosome Segregation, Mitotic Checkpoint Function, and Kinetochore Assembly. Mol. Biol. Cell 18: 2155-2168 [Abstract] [Full Text]  
  • Maddox, P. S., Portier, N., Desai, A., Oegema, K. (2006). Molecular analysis of mitotic chromosome condensation using a quantitative time-resolved fluorescence microscopy assay. Proc. Natl. Acad. Sci. USA 103: 15097-15102 [Abstract] [Full Text]  
  • Oliveira, R. A., Coelho, P. A., Sunkel, C. E. (2005). The Condensin I Subunit Barren/CAP-H Is Essential for the Structural Integrity of Centromeric Heterochromatin during Mitosis. Mol. Cell. Biol. 25: 8971-8984 [Abstract] [Full Text]  
  • Losada, A., Hirano, T. (2005). Dynamic molecular linkers of the genome: the first decade of SMC proteins. Genes Dev. 19: 1269-1287 [Abstract] [Full Text]  


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