This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jimeno, S.
Right arrow Articles by Aguilera, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jimeno, S.
Right arrow Articles by Aguilera, A.

Next Article 

Molecular and Cellular Biology, June 2006, p. 4387-4398, Vol. 26, No. 12
0270-7306/06/$08.00+0     doi:10.1128/MCB.00234-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Tho1, a Novel hnRNP, and Sub2 Provide Alternative Pathways for mRNP Biogenesis in Yeast THO Mutants{dagger}

Sonia Jimeno, Rosa Luna, María García-Rubio, and Andrés Aguilera*

Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain

Received 4 October 2005/ Returned for modification 5 December 2005/ Accepted 21 March 2006

THO is a protein complex that functions in cotranscriptional mRNP formation. Yeast THO1 and SUB2 (Saccharomyces cerevisiae) were identified as multicopy suppressors of the expression defects of the hpr1{Delta} mutant of THO. Here we show that multicopy THO1 suppresses the mRNA accumulation and export defects and the hyperrecombination phenotype of THO mutants but not those of sub2{Delta}, thp1{Delta}, or spt4{Delta}. Similarly, Sub2 overexpression suppresses the RNA export defect of hpr1{Delta}. Tho1 is a conserved RNA binding nuclear protein that specifically binds to transcribed chromatin in a THO- and RNA-dependent manner and genetically interacts with the shuttling hnRNP Nab2. The ability of Tho1 to suppress hpr1{Delta} resides in its C-terminal half, which contains the RNA binding activity and is located after a SAP/SAF (scaffold-associated protein/scaffold-associated factor) domain. Altogether, these results suggest that Tho1 is an hnRNP that, similarly to Sub2, assembles onto the nascent mRNA during transcription and participates in mRNP biogenesis and export. Overexpression of Tho1 or Sub2 may provide alternative ways for mRNP formation and export in the absence of a functional THO complex.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain. Phone: 34 954 557107. Fax: 34 954 5571204. E-mail: aguilo{at}us.es.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, June 2006, p. 4387-4398, Vol. 26, No. 12
0270-7306/06/$08.00+0     doi:10.1128/MCB.00234-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gomez-Gonzalez, B., Aguilera, A. (2009). R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX. Nucleic Acids Res 37: 4315-4321 [Abstract] [Full Text]  
  • Rollenhagen, C., Hodge, C. A., Cole, C. N. (2007). Following Temperature Stress, Export of Heat Shock mRNA Occurs Efficiently in Cells with Mutations in Genes Normally Important for mRNA Export. Eukaryot Cell 6: 505-513 [Abstract] [Full Text]  
  • Huertas, P., Garcia-Rubio, M. L., Wellinger, R. E., Luna, R., Aguilera, A. (2006). An hpr1 Point Mutation That Impairs Transcription and mRNP Biogenesis without Increasing Recombination. Mol. Cell. Biol. 26: 7451-7465 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»