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Molecular and Cellular Biology, June 2006, p. 4519-4528, Vol. 26, No. 12
0270-7306/06/$08.00+0     doi:10.1128/MCB.00409-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SUMO Modification Enhances p66-Mediated Transcriptional Repression of the Mi-2/NuRD Complex

Zihua Gong, Marc Brackertz, and Rainer Renkawitz*

Institute for Genetics, Justus Liebig University Giessen, Giessen, Germany

Received 9 March 2006/ Returned for modification 22 March 2006/ Accepted 24 March 2006

Human p66{alpha} and p66ß are two potent transcriptional repressors that interact with the methyl-CpG-binding domain proteins MBD2 and MBD3. An analysis of the molecular mechanisms mediating repression resulted in the identification of two major repression domains in p66{alpha} and one in p66ß. Both p66{alpha} and p66ß are SUMO-modified in vivo: p66{alpha} at two sites (Lys-30 and Lys-487) and p66ß at one site (Lys-33). Expression of SUMO1 enhanced the transcriptional repression activity of Gal-p66{alpha} and Gal-p66ß. Mutation of the SUMO modification sites or using a SUMO1 mutant or a dominant negative Ubc9 ligase resulted in a significant decrease of the transcriptional repression of p66{alpha} and p66ß. The Mi-2/NuRD components MBD3, RbAp46, RbAp48, and HDAC1 were found to bind to both p66{alpha} and p66ß in vivo. Most of the interactions were not affected by the SUMO site mutations in p66{alpha} or p66ß, with two exceptions. HDAC1 binding to p66{alpha} was lost in the case of a p66{alpha}K30R mutant, and RbAp46 binding was reduced in the case of a p66ßK33R mutant. These results suggest that interactions within the Mi-2/NuRD complex as well as optimal repression are mediated by SUMOylation.

* Corresponding author. Mailing address: Institute for Genetics, Justus Liebig University Giessen, Heinrich-Buff-Ring 58-62, D-35392 Giessen, Germany. Phone: 49 641 35460. Fax: 49 641 35469. E-mail: Rainer.renkawitz{at}gen.bio.uni-giessen.de.

Molecular and Cellular Biology, June 2006, p. 4519-4528, Vol. 26, No. 12
0270-7306/06/$08.00+0     doi:10.1128/MCB.00409-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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