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Molecular and Cellular Biology, July 2006, p. 5106-5119, Vol. 26, No. 13
0270-7306/06/$08.00+0     doi:10.1128/MCB.00101-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Receptor Protein Tyrosine Phosphatase {gamma} Is a Marker for Pyramidal Cells and Sensory Neurons in the Nervous System and Is Not Necessary for Normal Development

Smaragda Lamprianou,1 Nathalie Vacaresse,1 Yoshihisa Suzuki,2 Hamid Meziane,3 Joseph D. Buxbaum,4 Joseph Schlessinger,2 and Sheila Harroch1*

Institut Pasteur, Department of Neuroscience, 25 Rue du Dr. Roux, 75724 Paris, France,1 Yale University School of Medicine, Department of Pharmacology, P.O. Box 208066, New Haven, Connecticut 06520-8066,2 Mouse Clinical Institute, 67404 Illkirch Cedex, Strasbourg, France;,3 Mount Sinai School of Medicine, Departments of Psychiatry, Neuroscience, and Geriatrics and Adult Development, One Gustave Levy Place, New York, New York 100294

Received 17 January 2006/ Returned for modification 26 February 2006/ Accepted 15 April 2006

In order to gain insight into the biological role of receptor protein tyrosine phosphatase {gamma} (RPTP{gamma}), we have generated RPTP{gamma}-null mice. RPTP{gamma} was disrupted by insertion of the ß-galactosidase gene under the control of the RPTP{gamma} promoter. As the RPTP{gamma}-null mice did not exhibit any obvious phenotype, we made use of these mice to study RPTP{gamma} expression and thus shed light on potential biological functions of this phosphatase. Inspection of mouse embryos shows that RPTP{gamma} is expressed in a variety of tissues during embryogenesis. RPTP{gamma} is expressed in both embryonic and adult brains. Specifically, we detected RPTP{gamma} expression in cortical layers II and V and in the stratum pyramidale of the hippocampus, indicating that RPTP{gamma} is a marker for pyramidal neurons. Mixed primary culture of glial cells showed a lack of expression of RPTP{gamma} in astrocytes and a low expression of RPTP{gamma} in oligodendrocytes and in microglia. Interestingly, RPTP{gamma} expression was detected in all sensory organs, including the ear, nose, tongue, eye, and vibrissa follicles, suggesting a potential role of RPTP{gamma} in sensory neurons. An initial behavioral analysis showed minor changes in the RPTP{gamma}-null mice.

* Corresponding author. Mailing address: Institut Pasteur, Department of Neuroscience, 25 Rue du Dr. Roux, 75724 Paris, France. Phone: (33)140613424. Fax: (33)140613421. E-mail: sharroch{at}pasteur.fr.

Molecular and Cellular Biology, July 2006, p. 5106-5119, Vol. 26, No. 13
0270-7306/06/$08.00+0     doi:10.1128/MCB.00101-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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