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Molecular and Cellular Biology, August 2006, p. 5650-5662, Vol. 26, No. 15
0270-7306/06/$08.00+0     doi:10.1128/MCB.00313-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Hypomorphic Mutation of the TALE Gene Prep1 (pKnox1) Causes a Major Reduction of Pbx and Meis Proteins and a Pleiotropic Embryonic Phenotype

Elisabetta Ferretti ,1,{dagger},{ddagger} J. Carlos Villaescusa,3,{ddagger} Patrizia Di Rosa,1 Luis C. Fernandez-Diaz,2,3 Elena Longobardi,2,3 Roberta Mazzieri,1 Annarita Miccio,4 Nicola Micali,1 Licia Selleri,5 Giuliana Ferrari,2,4 and Francesco Blasi1,2,3*

Laboratory of Molecular Genetics, DIBIT, via Olgettina 58, 20132 Milano, Italy,1 Università Vita Salute San Raffaele, via Olgettina 58, 20132 Milano, Italy,2 IFOM, FIRC Institute of Molecular Oncology, via Adamello 16, Milano 20139, Italy,3 San Raffaele Telethon Institute for Gene Therapy, via Olgettina 58, 20132 Milano, Italy,4 Department of Cell and Developmental Biology, Weill Medical College, Cornell University, New York, New York 100215

Received 20 February 2006/ Returned for modification 15 April 2006/ Accepted 6 May 2006

The interaction of Prep1 and Pbx homeodomain transcription factors regulates their activity, nuclear localization, and likely, function in development. To understand the in vivo role of Prep1, we have analyzed an embryonic lethal hypomorphic mutant mouse (Prep1i/i). Prep1i/i embryos die at embryonic day 17.5 (E17.5) to birth with an overall organ hypoplasia, severe anemia, impaired angiogenesis, and eye anomalies, particularly in the lens and retina. The anemia correlates with delayed differentiation of erythroid progenitors and may be, at least in part, responsible for intrauterine death. At E14.5, Prep1 is present in fetal liver (FL) cMyb-positive cells, whose deficiency causes a marked hematopoietic phenotype. Prep1 is also localized to FL endothelial progenitors, consistent with the observed angiogenic phenotype. Likewise, at the same gestational day, Prep1 is present in the eye cells that bear Pax6, implicated in eye development. The levels of cMyb and Pax6 in FL and in the retina, respectively, are significantly decreased in Prep1i/i embryos, consistent with the hematopoietic and eye phenotypes. Concomitantly, Prep1 deficiency results in the overall decrease of protein levels of its related family member Meis1 and its partners Pbx1 and Pbx2. As both Prep1 and Meis interact with Pbx, the overall Prep1/Meis-Pbx DNA-binding activity is strongly reduced in whole Prep1i/i embryos and their organs. Our data indicate that Prep1 is an essential gene that acts upstream of and within a Pbx-Meis network that regulates multiple aspects of embryonic development.

* Corresponding author. Mailing address: Università Vita Salute San Raffaele, via Olgettina 58, 20132 Milano, Italy. Phone: 39 02 2643 4832. Fax: 39 02 2643 4844. E-mail: blasi.francesco{at}hsr.it.

{dagger} Present address: Department of Cell and Developmental Biology, Weill Medical College, Cornell University, New York, NY 10021.

{ddagger} These authors equally contributed to this work.

Molecular and Cellular Biology, August 2006, p. 5650-5662, Vol. 26, No. 15
0270-7306/06/$08.00+0     doi:10.1128/MCB.00313-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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