This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guelman, S.
Right arrow Articles by Workman, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guelman, S.
Right arrow Articles by Workman, J. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2006, p. 7178-7189, Vol. 26, No. 19
0270-7306/06/$08.00+0     doi:10.1128/MCB.00130-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The Essential Gene wda Encodes a WD40 Repeat Subunit of Drosophila SAGA Required for Histone H3 Acetylation

Sebastián Guelman, Tamaki Suganuma, Laurence Florens, Vikki Weake, Selene K. Swanson, Michael P. Washburn, Susan M. Abmayr, and Jerry L. Workman*

Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, Missouri 64110

Received 21 January 2006/ Returned for modification 3 March 2006/ Accepted 17 July 2006

Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in organisms as diverse as Saccharomyces cerevisiae, humans, and flies. For example, the well-studied yeast SAGA is related to three mammalian complexes. We previously identified Drosophila melanogaster orthologues of yeast SAGA components Ada2, Ada3, Spt3, and Tra1 and demonstrated that they associate with dGcn5 in a high-molecular-weight complex. To better understand the function of Drosophila SAGA (dSAGA), we sought to affinity purify and characterize this complex in more detail. A proteomic approach led to the identification of an orthologue of the yeast protein Ada1 and the novel protein encoded by CG4448, referred to as WDA (will decrease acetylation). Embryos lacking both alleles of the wda gene exhibited reduced levels of histone H3 acetylation and could not develop into adult flies. Our results point to a critical function of dSAGA and histone acetylation during Drosophila development.

* Corresponding author. Mailing address: Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110. Phone: (816) 926-4312. Fax: (816) 926-4692. E-mail: jlw{at}stowers-institute.org.

Molecular and Cellular Biology, October 2006, p. 7178-7189, Vol. 26, No. 19
0270-7306/06/$08.00+0     doi:10.1128/MCB.00130-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Guelman, S., Kozuka, K., Mao, Y., Pham, V., Solloway, M. J., Wang, J., Wu, J., Lill, J. R., Zha, J. (2009). The Double-Histone-Acetyltransferase Complex ATAC Is Essential for Mammalian Development. Mol. Cell. Biol. 29: 1176-1188 [Abstract] [Full Text]  
  • Grau, B., Popescu, C., Torroja, L., Ortuno-Sahagun, D., Boros, I., Ferrus, A. (2008). Transcriptional Adaptor ADA3 of Drosophila melanogaster Is Required for Histone Modification, Position Effect Variegation, and Transcription. Mol. Cell. Biol. 28: 376-385 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»