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Molecular and Cellular Biology, November 2006, p. 7858-7870, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00870-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cotranscriptional Recruitment to the mRNA Export Receptor Mex67p Contributes to Nuclear Pore Anchoring of Activated Genes{triangledown}

Guennaelle Dieppois,{dagger} Nahid Iglesias,{dagger} and Françoise Stutz*

Department of Cell Biology, Sciences III, 30 Quai E. Ansermet, 1211 Geneva 4, Switzerland

Received 16 May 2006/ Returned for modification 14 June 2006/ Accepted 24 August 2006

Transcription activation of some Saccharomyces cerevisiae genes is paralleled by their repositioning to the nuclear periphery, but the mechanism underlying gene anchoring is poorly defined. We show that the nuclear pore complex-associated Mlp1p and the shuttling mRNA export receptor Mex67p contribute to the stable association of the activated GAL10 and HSP104 genes with the nuclear periphery. However, we find no obligatory link between gene positioning and gene expression. Furthermore, gene anchoring correlates with the cotranscriptional recruitment of Mex67p to transcribing genes. Notably, the association of Mex67p with chromatin is not mediated by RNA. Interestingly, a mutant GAL2 gene lacking the coding region is still able to recruit Mex67p upon transcriptional activation and to relocate to the nuclear periphery. Together these data suggest that, at least for GAL2, nascent messenger ribonucleoprotein does not play a major role in gene anchoring and that the early recruitment of Mex67p contributes to gene repositioning by virtue of an RNA-independent process.

* Corresponding author. Mailing address: Department of Cell Biology, Sciences III, 30 Quai E. Ansermet, 1211 Geneva 4, Switzerland. Phone: 0041 22 379 67 29. Fax: 0041 22 379 6442. E-mail: Francoise.Stutz{at}cellbio.unige.ch.

{triangledown} Published ahead of print on 5 September 2006.

{dagger} These authors contributed equally to this work.

Molecular and Cellular Biology, November 2006, p. 7858-7870, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00870-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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