This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hossain, A.
Right arrow Articles by Saunders, G. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hossain, A.
Right arrow Articles by Saunders, G. F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, November 2006, p. 8191-8201, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00242-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mir-17-5p Regulates Breast Cancer Cell Proliferation by Inhibiting Translation of AIB1 mRNA{triangledown}

Anwar Hossain,1* Macus T. Kuo,2 and Grady F. Saunders1,{dagger}

Department of Biochemistry and Molecular Biology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030,1 Department of Molecular Pathology, The University of Texas M. D. Anderson Cancer Center, 7435 Fannin Street, Houston, Texas 770542

Received 8 February 2006/ Returned for modification 3 April 2006/ Accepted 15 August 2006

MicroRNAs are an extensive family of ~22-nucleotide-long noncoding RNAs expressed in a wide range of eukaryotes, including humans, and they are important in development and disease. We found that microRNA Mir-17-5p has extensive complementarity to the mRNA of AIB1 (named for "amplified in breast cancer 1"). Cell culture experiments showed that AIB1 expression was downregulated by Mir-17-5p, primarily through translational inhibition. Expression of Mir-17-5p was low in breast cancer cell lines. We also found that downregulation of AIB1 by Mir-17-5p resulted in decreased estrogen receptor-mediated, as well as estrogen receptor-independent, gene expression and decreased proliferation of breast cancer cells. Mir-17-5p also completely abrogated the insulin-like growth factor 1-mediated, anchorage-independent growth of breast cancer cells. Our results reveal that Mir-17-5p has a role as a tumor suppressor in breast cancer cells.

* Corresponding author. Mailing address: Department of Molecular Pathology, Unit 89, The University of Texas M. D. Anderson Cancer Center, 7435 Fannin Street, Houston, TX 77054. Phone: (713) 834-6079. Fax: (713) 834-6084. E-mail: ahossain{at}mdanderson.org.

{triangledown} Published ahead of print on 28 August 2006.

{dagger} Deceased.

Molecular and Cellular Biology, November 2006, p. 8191-8201, Vol. 26, No. 21
0270-7306/06/$08.00+0     doi:10.1128/MCB.00242-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Castellano, L., Giamas, G., Jacob, J., Coombes, R. C., Lucchesi, W., Thiruchelvam, P., Barton, G., Jiao, L. R., Wait, R., Waxman, J., Hannon, G. J., Stebbing, J. (2009). The estrogen receptor-{alpha}-induced microRNA signature regulates itself and its transcriptional response. Proc. Natl. Acad. Sci. USA 106: 15732-15737 [Abstract] [Full Text]  
  • Tan, L. P., Seinen, E., Duns, G., de Jong, D., Sibon, O. C. M., Poppema, S., Kroesen, B.-J., Kok, K., van den Berg, A. (2009). A high throughput experimental approach to identify miRNA targets in human cells. Nucleic Acids Res 0: gkp715v1-gkp715 [Abstract] [Full Text]  
  • Wang, Y., Rathinam, R., Walch, A., Alahari, S. K. (2009). ST14 (Suppression of Tumorigenicity 14) Gene Is a Target for miR-27b, and the Inhibitory Effect of ST14 on Cell Growth Is Independent of miR-27b Regulation. J. Biol. Chem. 284: 23094-23106 [Abstract] [Full Text]  
  • Sotiropoulou, G., Pampalakis, G., Lianidou, E., Mourelatos, Z. (2009). Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell. RNA 15: 1443-1461 [Abstract] [Full Text]  
  • Adams, B. D., Cowee, D. M., White, B. A. (2009). The Role of miR-206 in the Epidermal Growth Factor (EGF) Induced Repression of Estrogen Receptor-{alpha} (ER{alpha}) Signaling and a Luminal Phenotype in MCF-7 Breast Cancer Cells. Mol. Endocrinol. 23: 1215-1230 [Abstract] [Full Text]  
  • Bhat-Nakshatri, P., Wang, G., Collins, N. R., Thomson, M. J., Geistlinger, T. R., Carroll, J. S., Brown, M., Hammond, S., Srour, E. F., Liu, Y., Nakshatri, H. (2009). Estradiol-regulated microRNAs control estradiol response in breast cancer cells. Nucleic Acids Res 37: 4850-4861 [Abstract] [Full Text]  
  • Wickramasinghe, N. S., Manavalan, T. T., Dougherty, S. M., Riggs, K. A., Li, Y., Klinge, C. M. (2009). Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells. Nucleic Acids Res 37: 2584-2595 [Abstract] [Full Text]  
  • Khoshnaw, S M, Green, A R, Powe, D G, Ellis, I O (2009). MicroRNA involvement in the pathogenesis and management of breast cancer. J. Clin. Pathol. 62: 422-428 [Abstract] [Full Text]  
  • Bartels, C. L., Tsongalis, G. J. (2009). MicroRNAs: Novel Biomarkers for Human Cancer. Clin. Chem. 55: 623-631 [Abstract] [Full Text]  
  • Visone, R., Croce, C. M. (2009). MiRNAs and Cancer. Am. J. Pathol. 174: 1131-1138 [Abstract] [Full Text]  
  • Inomata, M., Tagawa, H., Guo, Y.-M., Kameoka, Y., Takahashi, N., Sawada, K. (2009). MicroRNA-17-92 down-regulates expression of distinct targets in different B-cell lymphoma subtypes. Blood 113: 396-402 [Abstract] [Full Text]  
  • Xiao, F., Zuo, Z., Cai, G., Kang, S., Gao, X., Li, T. (2009). miRecords: an integrated resource for microRNA-target interactions. Nucleic Acids Res 37: D105-D110 [Abstract] [Full Text]  
  • Toloubeydokhti, T., Qun Pan, , Xiaoping Luo, , Bukulmez, O., Chegini, N. (2008). The Expression and Ovarian Steroid Regulation of Endometrial Micro-RNAs. Reproductive Sciences 15: 993-1001 [Abstract]  
  • Yu, Z., Wang, C., Wang, M., Li, Z., Casimiro, M. C., Liu, M., Wu, K., Whittle, J., Ju, X., Hyslop, T., McCue, P., Pestell, R. G. (2008). A cyclin D1/microRNA 17/20 regulatory feedback loop in control of breast cancer cell proliferation. JCB 182: 509-517 [Abstract] [Full Text]  
  • Kondo, N., Toyama, T., Sugiura, H., Fujii, Y., Yamashita, H. (2008). miR-206 Expression Is Down-regulated in Estrogen Receptor {alpha}-Positive Human Breast Cancer. Cancer Res. 68: 5004-5008 [Abstract] [Full Text]  
  • Sengupta, S., den Boon, J. A., Chen, I-H., Newton, M. A., Stanhope, S. A., Cheng, Y.-J., Chen, C.-J., Hildesheim, A., Sugden, B., Ahlquist, P. (2008). MicroRNA 29c is down-regulated in nasopharyngeal carcinomas, up-regulating mRNAs encoding extracellular matrix proteins. Proc. Natl. Acad. Sci. USA 105: 5874-5878 [Abstract] [Full Text]  
  • Ivanovska, I., Ball, A. S., Diaz, R. L., Magnus, J. F., Kibukawa, M., Schelter, J. M., Kobayashi, S. V., Lim, L., Burchard, J., Jackson, A. L., Linsley, P. S., Cleary, M. A. (2008). MicroRNAs in the miR-106b Family Regulate p21/CDKN1A and Promote Cell Cycle Progression. Mol. Cell. Biol. 28: 2167-2174 [Abstract] [Full Text]  
  • Sampson, V. B., Rong, N. H., Han, J., Yang, Q., Aris, V., Soteropoulos, P., Petrelli, N. J., Dunn, S. P., Krueger, L. J. (2007). MicroRNA Let-7a Down-regulates MYC and Reverts MYC-Induced Growth in Burkitt Lymphoma Cells. Cancer Res. 67: 9762-9770 [Abstract] [Full Text]  
  • Tang, X., Gal, J., Zhuang, X., Wang, W., Zhu, H., Tang, G. (2007). A simple array platform for microRNA analysis and its application in mouse tissues. RNA 13: 1803-1822 [Abstract] [Full Text]  
  • Zhang, W., Dahlberg, J. E., Tam, W. (2007). MicroRNAs in Tumorigenesis: A Primer. Am. J. Pathol. 171: 728-738 [Abstract] [Full Text]  
  • Dai, R., Phillips, R. A., Ahmed, S. A. (2007). Despite Inhibition of Nuclear Localization of NF-{kappa}B p65, c-Rel, and RelB, 17-beta Estradiol Up-Regulates NF-{kappa}B Signaling in Mouse Splenocytes: The Potential Role of Bcl-3. J. Immunol. 179: 1776-1783 [Abstract] [Full Text]  
  • Landais, S., Landry, S., Legault, P., Rassart, E. (2007). Oncogenic Potential of the miR-106-363 Cluster and Its Implication in Human T-Cell Leukemia. Cancer Res. 67: 5699-5707 [Abstract] [Full Text]  
  • Adams, B. D., Furneaux, H., White, B. A. (2007). The Micro-Ribonucleic Acid (miRNA) miR-206 Targets the Human Estrogen Receptor-{alpha} (ER{alpha}) and Represses ER{alpha} Messenger RNA and Protein Expression in Breast Cancer Cell Lines. Mol. Endocrinol. 21: 1132-1147 [Abstract] [Full Text]  
  • Blenkiron, C., Miska, E. A. (2007). miRNAs in cancer: approaches, aetiology, diagnostics and therapy. Hum Mol Genet 16: R106-R113 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»