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Molecular and Cellular Biology, December 2006, p. 9291-9301, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01183-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evolutionarily Conserved Role of Nucleostemin: Controlling Proliferation of Stem/Progenitor Cells during Early Vertebrate Development{triangledown} ,{dagger}

Chantal Beekman,1 Massimo Nichane,2 Sarah De Clercq,1 Marion Maetens,1 Thomas Floss,3 Wolfgang Wurst,3 Eric Bellefroid,2 and Jean-Christophe Marine1*

Laboratory for Molecular Cancer Biology, Flanders Interuniversity Institute for Biotechnology (VIB) and University of Gent, B-9052 Ghent, Belgium,1 Unit of Molecular Embryology, Free University of Brussels (ULB-IBMM), Gosselies, Belgium,2 GSF-Institute of Mammalian Genetics, Neuherberg, Germany3

Received 29 June 2006/ Accepted 15 September 2006

Nucleostemin (NS) is a putative GTPase expressed preferentially in the nucleoli of neuronal and embryonic stem cells and several cancer cell lines. Transfection and knockdown studies indicated that NS controls the proliferation of these cells by interacting with the p53 tumor suppressor protein and regulating its activity. To assess the physiological role of NS in vivo, we generated a mutant mouse line with a specific gene trap event that inactivates the NS allele. The corresponding NS–/– embryos died around embryonic day 4. Analyses of NS mutant blastocysts indicated that NS is not required to maintain pluripotency, nucleolar integrity, or survival of the embryonic stem cells. However, the homozygous mutant blastocysts failed to enter S phase even in the absence of functional p53. Haploid insufficiency of NS in mouse embryonic fibroblasts leads to decreased cell proliferation. NS also functions in early amphibian development to control cell proliferation of neural progenitor cells. Our results show that NS has a unique ability, derived from an ancestral function, to control the proliferation rate of stem/progenitor cells in vivo independently of p53.

* Corresponding author. Mailing address: Laboratory for Molecular Cancer Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Technologiepark, 927, B-9052 Ghent, Belgium. Phone: 32 9 3313640. Fax: 32 9 3313516. E-mail: chris.marine{at}dmbr.ugent.be.

{triangledown} Published ahead of print on 25 September 2006.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, December 2006, p. 9291-9301, Vol. 26, No. 24
0270-7306/06/$08.00+0     doi:10.1128/MCB.01183-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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