This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iizuka, M.
Right arrow Articles by Smith, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iizuka, M.
Right arrow Articles by Smith, M. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 2006, p. 1098-1108, Vol. 26, No. 3
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.3.1098-1108.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of Replication Licensing by Acetyltransferase Hbo1

Masayoshi Iizuka,1,{dagger} Tomoko Matsui,2,{dagger} Haruhiko Takisawa,2* and M. Mitchell Smith1*

Department of Microbiology, University of Virginia Health System, Charlottesville, Virginia 22908,1 Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan2

Received 26 July 2005/ Returned for modification 20 August 2005/ Accepted 14 November 2005

The initiation of DNA replication is tightly regulated in eukaryotic cells to ensure that the genome is precisely duplicated once and only once per cell cycle. This is accomplished by controlling the assembly of a prereplicative complex (pre-RC) which involves the sequential binding to replication origins of the origin recognition complex (ORC), Cdc6/Cdc18, Cdt1, and the minichromosome maintenance complex (Mcm2-Mcm7, or Mcm2-7). Several mechanisms of pre-RC regulation are known, including ATP utilization, cyclin-dependent kinase levels, protein turnover, and Cdt1 binding by geminin. Histone acetylation may also affect the initiation of DNA replication, but at present neither the enzymes nor the steps involved are known. Here, we show that Hbo1, a member of the MYST histone acetyltransferase family, is a previously unrecognized positive regulatory factor for pre-RC assembly. When Hbo1 expression was inhibited in human cells, Mcm2-7 failed to associate with chromatin even though ORC and Cdc6 loading was normal. When Xenopus egg extracts were immunodepleted of Xenopus Hbo1 (XHbo1), chromatin binding of Mcm2-7 was lost, and DNA replication was abolished. The binding of Mcm2-7 to chromatin in XHbo1-depleted extracts could be restored by the addition of recombinant Cdt1.

* Corresponding author. Mailing address for M. Mitchell Smith: Department of Microbiology, University of Virginia Health System, P.O. Box 800734, Charlottesville, VA 22908-0734. Phone: (434) 924-2669. Fax: (434) 982-1071. E-mail: mms7r{at}virginia.edu. Mailing address for Haruhiko Takisawa: Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan. Phone: 81-6-6850-6762. Fax: 81-6-6850-6762. E-mail: takisawa{at}bio.sci.osaka-u.ac.jp.

{dagger} M.I. and T.M. contributed equally to this work.

Molecular and Cellular Biology, February 2006, p. 1098-1108, Vol. 26, No. 3
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.3.1098-1108.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jiang, Y., Lucas, I., Young, D. J., Davis, E. M., Karrison, T., Rest, J. S., Le Beau, M. M. (2009). Common fragile sites are characterized by histone hypoacetylation. Hum Mol Genet 18: 4501-4512 [Abstract] [Full Text]  
  • Houchens, C. R., Lu, W., Chuang, R.-Y., Frattini, M. G., Fuller, A., Simancek, P., Kelly, T. J. (2008). Multiple Mechanisms Contribute to Schizosaccharomyces pombe Origin Recognition Complex-DNA Interactions. J. Biol. Chem. 283: 30216-30224 [Abstract] [Full Text]  
  • Foy, R. L., Song, I. Y., Chitalia, V. C., Cohen, H. T., Saksouk, N., Cayrou, C., Vaziri, C., Cote, J., Panchenko, M. V. (2008). Role of Jade-1 in the Histone Acetyltransferase (HAT) HBO1 Complex. J. Biol. Chem. 283: 28817-28826 [Abstract] [Full Text]  
  • Miotto, B., Struhl, K. (2008). HBO1 histone acetylase is a coactivator of the replication licensing factor Cdt1. Genes Dev. 22: 2633-2638 [Abstract] [Full Text]  
  • Zou, Y., Bi, X. (2008). Positive roles of SAS2 in DNA replication and transcriptional silencing in yeast. Nucleic Acids Res 36: 5189-5200 [Abstract] [Full Text]  
  • Gomez, E. B., Nugent, R. L., Laria, S., Forsburg, S. L. (2008). Schizosaccharomyces pombe Histone Acetyltransferase Mst1 (KAT5) Is an Essential Protein Required for Damage Response and Chromosome Segregation. Genetics 179: 757-771 [Abstract] [Full Text]  
  • Wu, Z.-Q., Liu, X. (2008). Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing. Proc. Natl. Acad. Sci. USA 105: 1919-1924 [Abstract] [Full Text]  
  • Johmura, Y., Osada, S., Nishizuka, M., Imagawa, M. (2008). FAD24 Acts in Concert with Histone Acetyltransferase HBO1 to Promote Adipogenesis by Controlling DNA Replication. J. Biol. Chem. 283: 2265-2274 [Abstract] [Full Text]  
  • Iizuka, M., Sarmento, O. F., Sekiya, T., Scrable, H., Allis, C. D., Smith, M. M. (2008). Hbo1 Links p53-Dependent Stress Signaling to DNA Replication Licensing. Mol. Cell. Biol. 28: 140-153 [Abstract] [Full Text]  
  • Tardat, M., Murr, R., Herceg, Z., Sardet, C., Julien, E. (2007). PR-Set7 dependent lysine methylation ensures genome replication and stability through S phase. JCB 179: 1413-1426 [Abstract] [Full Text]  
  • Lottersberger, F., Panza, A., Lucchini, G., Longhese, M. P. (2007). Functional and Physical Interactions between Yeast 14-3-3 Proteins, Acetyltransferases, and Deacetylases in Response to DNA Replication Perturbations. Mol. Cell. Biol. 27: 3266-3281 [Abstract] [Full Text]  
  • Dammer, E. B., Leon, A., Sewer, M. B. (2007). Coregulator Exchange and Sphingosine-Sensitive Cooperativity of Steroidogenic Factor-1, General Control Nonderepressed 5, p54, and p160 Coactivators Regulate Cyclic Adenosine 3',5'-Monophosphate-Dependent Cytochrome P450c17 Transcription Rate. Mol. Endocrinol. 21: 415-438 [Abstract] [Full Text]  
  • Fukuda, H., Sano, N., Muto, S., Horikoshi, M. (2006). Simple histone acetylation plays a complex role in the regulation of gene expression.. Brief Funct Genomic Proteomic 5: 190-208 [Abstract] [Full Text]  
  • Georgiakaki, M., Chabbert-Buffet, N., Dasen, B., Meduri, G., Wenk, S., Rajhi, L., Amazit, L., Chauchereau, A., Burger, C. W., Blok, L. J., Milgrom, E., Lombes, M., Guiochon-Mantel, A., Loosfelt, H. (2006). Ligand-Controlled Interaction of Histone Acetyltransferase Binding to ORC-1 (HBO1) with the N-Terminal Transactivating Domain of Progesterone Receptor Induces Steroid Receptor Coactivator 1-Dependent Coactivation of Transcription. Mol. Endocrinol. 20: 2122-2140 [Abstract] [Full Text]  
  • Ghosh, M., Kemp, M., Liu, G., Ritzi, M., Schepers, A., Leffak, M. (2006). Differential Binding of Replication Proteins across the Human c-myc Replicator.. Mol. Cell. Biol. 26: 5270-5283 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»