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Molecular and Cellular Biology, March 2006, p. 2029-2036, Vol. 26, No. 6
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.6.2029-2036.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Novel RNA Binding Domain in Tetrahymena Telomerase p65 Initiates Hierarchical Assembly of Telomerase Holoenzyme

Catherine M. O'Connor and Kathleen Collins*

Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3204

Received 2 November 2005/ Returned for modification 30 November 2005/ Accepted 31 December 2005

Telomerase reverse transcriptase (TERT) and telomerase RNA (TER) assemble as part of a holoenzyme that synthesizes telomeric repeats at chromosome ends. Genetic approaches have identified proteins that are required for in vivo association of TERT and TER, including the Tetrahymena telomerase holoenzyme protein p65. Here, we use quantitative assays to define the mechanisms underlying p65 function in holoenzyme biogenesis. We demonstrate that four modules of p65 contribute affinity for TER, including a C-terminal domain that recognizes the conserved dinucleotide bulge of central stem IV. This C-terminal domain is necessary and sufficient for p65's function in enhancing the recruitment of TERT to TER. Finally, we show that p65 and TERT assemble on TER with hierarchical rather than cooperative binding. These findings elucidate an extensive network of p65-TER recognition specificity and define a novel p65 RNA binding domain that initiates telomerase holoenyzme biogenesis.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720-3204. Phone: (510) 643-1598. Fax: (510) 643-6334. E-mail: kcollins{at}berkeley.edu.

Molecular and Cellular Biology, March 2006, p. 2029-2036, Vol. 26, No. 6
0022-538X/06/$08.00+0     doi:10.1128/MCB.26.6.2029-2036.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Witkin, K. L., Prathapam, R., Collins, K. (2007). Positive and Negative Regulation of Tetrahymena Telomerase Holoenzyme. Mol. Cell. Biol. 27: 2074-2083 [Abstract] [Full Text]  
  • Mozdy, A. D., Cech, T. R. (2006). Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency. RNA 12: 1721-1737 [Abstract] [Full Text]  
  • Richards, R. J., Wu, H., Trantirek, L., O'Connor, C. M., Collins, K., Feigon, J. (2006). Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for function. RNA 12: 1475-1485 [Abstract] [Full Text]  


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