This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Qiu, H.
Right arrow Articles by Hinnebusch, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Qiu, H.
Right arrow Articles by Hinnebusch, A. G.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2006, p. 3135-3148, Vol. 26, No. 8
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.8.3135-3148.2006

The Spt4p Subunit of Yeast DSIF Stimulates Association of the Paf1 Complex with Elongating RNA Polymerase II

Hongfang Qiu, Cuihua Hu, Chi-Ming Wong, and Alan G. Hinnebusch*

Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892

Received 8 December 2005/ Accepted 12 January 2006

The Paf1 complex (Paf1C) interacts with RNA polymerase II (Pol II) and promotes histone methylation of transcribed coding sequences, but the mechanism of Paf1C recruitment is unknown. We show that Paf1C is not recruited directly by the activator Gcn4p but is dependent on preinitiation complex assembly and Ser5 carboxy-terminal domain phosphorylation for optimal association with ARG1 coding sequences. Importantly, Spt4p is required for Paf1C occupancy at ARG1 (and other genes) and for Paf1C association with Ser5-phosphorylated Pol II in cell extracts, whereas Spt4p-Pol II association is independent of Paf1C. Since spt4{Delta} does not reduce levels of Pol II at ARG1, Ser5 phosphorylation, or Paf1C expression, it appears that Spt4p (or its partner in DSIF, Spt5p) provides a platform on Pol II for recruiting Paf1C following Ser5 phosphorylation and promoter clearance. spt4{Delta} reduces trimethylation of Lys4 on histone H3, demonstrating a new role for yeast DSIF in promoting a Paf1C-dependent function in elongation.

* Corresponding author. Mailing address: NIH, Building 6A, Room B1A-13, Bethesda, MD 20892. Phone: (301) 496-4480. Fax: (301) 496-6828. E-mail: ahinnebusch{at}nih.gov.

Molecular and Cellular Biology, April 2006, p. 3135-3148, Vol. 26, No. 8
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.8.3135-3148.2006




This article has been cited by other articles:

  • Liu, Y., Warfield, L., Zhang, C., Luo, J., Allen, J., Lang, W. H., Ranish, J., Shokat, K. M., Hahn, S. (2009). Phosphorylation of the Transcription Elongation Factor Spt5 by Yeast Bur1 Kinase Stimulates Recruitment of the PAF Complex. Mol. Cell. Biol. 29: 4852-4863 [Abstract] [Full Text]  
  • Schwer, B., Schneider, S., Pei, Y., Aronova, A., Shuman, S. (2009). Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex. RNA 15: 1241-1250 [Abstract] [Full Text]  
  • Zhou, K., Kuo, W. H. W., Fillingham, J., Greenblatt, J. F. (2009). Control of transcriptional elongation and cotranscriptional histone modification by the yeast BUR kinase substrate Spt5. Proc. Natl. Acad. Sci. USA 106: 6956-6961 [Abstract] [Full Text]  
  • Zhang, Y., Sikes, M. L., Beyer, A. L., Schneider, D. A. (2009). The Paf1 complex is required for efficient transcription elongation by RNA polymerase I. Proc. Natl. Acad. Sci. USA 106: 2153-2158 [Abstract] [Full Text]  
  • Pascual-Garcia, P., Govind, C. K., Queralt, E., Cuenca-Bono, B., Llopis, A., Chavez, S., Hinnebusch, A. G., Rodriguez-Navarro, S. (2008). Sus1 is recruited to coding regions and functions during transcription elongation in association with SAGA and TREX2. Genes Dev. 22: 2811-2822 [Abstract] [Full Text]  
  • He, Q., Battistella, L., Morse, R. H. (2008). Mediator Requirement Downstream of Chromatin Remodeling during Transcriptional Activation of CHA1 in Yeast. J. Biol. Chem. 283: 5276-5286 [Abstract] [Full Text]  
  • Hershman, S. G., Chen, Q., Lee, J. Y., Kozak, M. L., Yue, P., Wang, L.-S., Johnson, F. B. (2008). Genomic distribution and functional analyses of potential G-quadruplex-forming sequences in Saccharomyces cerevisiae. Nucleic Acids Res 36: 144-156 [Abstract] [Full Text]  
  • Nyswaner, K. M., Checkley, M. A., Yi, M., Stephens, R. M., Garfinkel, D. J. (2008). Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis. Genetics 178: 197-214 [Abstract] [Full Text]  
  • Wong, C.-M., Qiu, H., Hu, C., Dong, J., Hinnebusch, A. G. (2007). Yeast Cap Binding Complex Impedes Recruitment of Cleavage Factor IA to Weak Termination Sites. Mol. Cell. Biol. 27: 6520-6531 [Abstract] [Full Text]  
  • Warner, M. H., Roinick, K. L., Arndt, K. M. (2007). Rtf1 Is a Multifunctional Component of the Paf1 Complex That Regulates Gene Expression by Directing Cotranscriptional Histone Modification. Mol. Cell. Biol. 27: 6103-6115 [Abstract] [Full Text]  
  • Cui, X., Fan, B., Scholz, J., Chen, Z. (2007). Roles of Arabidopsis Cyclin-Dependent Kinase C Complexes in Cauliflower Mosaic Virus Infection, Plant Growth, and Development. Plant Cell 19: 1388-1402 [Abstract] [Full Text]  
  • Reyes-Reyes, M., Hampsey, M. (2007). Role for the Ssu72 C-Terminal Domain Phosphatase in RNA Polymerase II Transcription Elongation. Mol. Cell. Biol. 27: 926-936 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»