This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Varon, C.
Right arrow Articles by Génot, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Varon, C.
Right arrow Articles by Génot, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2006, p. 3582-3594, Vol. 26, No. 9
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.9.3582-3594.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Transforming Growth Factor ß Induces Rosettes of Podosomes in Primary Aortic Endothelial Cells{dagger}

Christine Varon,1,2 Florence Tatin,1,2 Violaine Moreau,1,2 Ellen Van Obberghen-Schilling,3 Samantha Fernandez-Sauze,3 Edith Reuzeau,1,2 Ijsbrand Kramer,1,2 and Elisabeth Génot1,2*

INSERM Unité 441, University Victor Segalen Bordeaux 2, Bordeaux, France,1 European Institute of Chemistry and Biology, University of Bordeaux I, Talence, France,2 Institute of Signalling, Developmental Biology and Cancer Research, CNRS UMR6543, Centre A, Lacassagne, Nice, France3

Received 11 October 2005/ Returned for modification 28 November 2005/ Accepted 14 February 2006

Cytoskeletal rearrangements are central to endothelial cell physiology and are controlled by soluble factors, matrix proteins, cell-cell interactions, and mechanical forces. We previously reported that aortic endothelial cells can rearrange their cytoskeletons into complex actin-based structures called podosomes when a constitutively active mutant of Cdc42 is expressed. We now report that transforming growth factor beta (TGF-ß) promotes podosome formation in primary aortic endothelial cells. TGF-ß-induced podosomes assembled together into large ring- or crescent-shaped structures. Their formation was dependent on protein synthesis and required functional Src, phosphatidylinositide 3-kinase, Cdc42, RhoA, and Smad signaling. MT1-MMP and metalloprotease 9 (MMP9), both upregulated by TGF-ß, were detected at sites of podosome formation, and MT1-MMP was found to be involved in the local degradation of extracellular matrix proteins beneath the podosomes and required for the invasion of collagen gels by endothelial cells. We propose that TGF-ß plays an important role in endothelial cell physiology by inducing the formation of podosomal structures endowed with metalloprotease activity that may contribute to arterial remodeling.

* Corresponding author. Mailing address: European Institute of Chemistry and Biology, 2 rue Robert Escarpit, 33600, Pessac, France. Phone: 33 (0) 5 40 00 30 56. Fax: 33 (0) 5 40 00 87 26. E-mail: e.genot{at}iecb.u-bordeaux.fr.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, May 2006, p. 3582-3594, Vol. 26, No. 9
0270-7306/06/$08.00+0     doi:10.1128/MCB.26.9.3582-3594.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Linder, S. (2009). Invadosomes at a glance. J. Cell Sci. 122: 3009-3013 [Full Text]  
  • Varon, C, Duriez, A, Lehours, P, Menard, A, Laye, S, Zerbib, F, Megraud, F, Laharie, D (2009). Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro. Gut 58: 629-635 [Abstract] [Full Text]  
  • Buschman, M. D., Bromann, P. A., Cejudo-Martin, P., Wen, F., Pass, I., Courtneidge, S. A. (2009). The Novel Adaptor Protein Tks4 (SH3PXD2B) Is Required for Functional Podosome Formation. Mol. Biol. Cell 20: 1302-1311 [Abstract] [Full Text]  
  • Wang, J., Taba, Y., Pang, J., Yin, G., Yan, C., Berk, B. C. (2009). GIT1 Mediates VEGF-Induced Podosome Formation in Endothelial Cells: Critical Role for PLC{gamma}. Arterioscler. Thromb. Vasc. Bio. 29: 202-208 [Abstract] [Full Text]  
  • Aga, M., Bradley, J. M., Keller, K. E., Kelley, M. J., Acott, T. S. (2008). Specialized Podosome- or Invadopodia-like Structures (PILS) for Focal Trabecular Meshwork Extracellular Matrix Turnover. IOVS 49: 5353-5365 [Abstract] [Full Text]  
  • Ouyang, M., Lu, S., Li, X.-Y., Xu, J., Seong, J., Giepmans, B. N. G., Shyy, J. Y.-J., Weiss, S. J., Wang, Y. (2008). Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase Activity in Live Cells by Fluorescence Resonance Energy Transfer Imaging. J. Biol. Chem. 283: 17740-17748 [Abstract] [Full Text]  
  • Desai, B., Ma, T., Chellaiah, M. A. (2008). Invadopodia and Matrix Degradation, a New Property of Prostate Cancer Cells during Migration and Invasion. J. Biol. Chem. 283: 13856-13866 [Abstract] [Full Text]  
  • Chabadel, A., Banon-Rodriguez, I., Cluet, D., Rudkin, B. B., Wehrle-Haller, B., Genot, E., Jurdic, P., Anton, I. M., Saltel, F. (2007). CD44 and beta3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts. Mol. Biol. Cell 18: 4899-4910 [Abstract] [Full Text]  
  • Malek, A. M., Xu, C., Kim, E. S., Alper, S. L. (2007). Hypertonicity triggers RhoA-dependent assembly of myosin-containing striated polygonal actin networks in endothelial cells. Am. J. Physiol. Cell Physiol. 292: C1645-C1659 [Abstract] [Full Text]  
  • Bajaj, M., Medina-Navarro, R., Suraamornkul, S., Meyer, C., DeFronzo, R. A., Mandarino, L. J. (2007). Paradoxical Changes in Muscle Gene Expression in Insulin-Resistant Subjects After Sustained Reduction in Plasma Free Fatty Acid Concentration. Diabetes 56: 743-752 [Abstract] [Full Text]  
  • Barbolina, M. V., Adley, B. P., Ariztia, E. V., Liu, Y., Stack, M. S. (2007). Microenvironmental Regulation of Membrane Type 1 Matrix Metalloproteinase Activity in Ovarian Carcinoma Cells via Collagen-induced EGR1 Expression. J. Biol. Chem. 282: 4924-4931 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»