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 Previous Article

Molecular and Cellular Biology, May 2007, p. 3881-3890, Vol. 27, No. 10
0270-7306/07/$08.00+0     doi:10.1128/MCB.02366-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

trans Autophosphorylation at DNA-Dependent Protein Kinase's Two Major Autophosphorylation Site Clusters Facilitates End Processing but Not End Joining{triangledown}

Katheryn Meek,1* Pauline Douglas,2 Xiaoping Cui,1 Qi Ding,1 and Susan P. Lees-Miller2

College of Veterinary Medicine and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan 48824,1 Department of Biochemistry & Molecular Biology and Department of Oncology, University of Calgary, Calgary, Alberta, Canada T2N4N12

Received 19 December 2006/ Returned for modification 14 January 2007/ Accepted 26 February 2007

Recent studies have established that DNA-dependent protein kinase (DNA-PK) undergoes a series of autophosphorylation events that facilitate successful completion of nonhomologous DNA end joining. Autophosphorylation at sites in two distinct clusters regulates DNA end access to DNA end-processing factors and to other DNA repair pathways. Autophosphorylation within the kinase's activation loop regulates kinase activity. Additional autophosphorylation events (as yet undefined) occur that mediate kinase dissociation. Here we provide the first evidence that autophosphorylation within the two major clusters (regulating end access) occurs in trans. Further, both UV-induced and double-strand break (DSB)-induced phosphorylation in the two major clusters is predominately autophosphorylation. Finally, we show that while autophosphorylation in trans on one of two synapsed DNA-PK complexes facilitates appropriate end processing, this is not sufficient to promote efficient end joining. This suggests that end joining in living cells requires additional phosphorylation events that either occur in cis or that occur on both sides of the DNA-PK synapse. These data support an emerging consensus that, via a series of autophosphorylation events, DNA-PK undergoes a sequence of conformational changes that promote efficient and appropriate repair of DSBs.

* Corresponding author. Mailing address: Michigan State University, 350 FST, East Lansing, MI 48824. Phone: (517) 432-9505. Fax: (517) 353-9004. E-mail: kmeek{at}msu.edu

{triangledown} Published ahead of print on 12 March 2007.

Molecular and Cellular Biology, May 2007, p. 3881-3890, Vol. 27, No. 10
0270-7306/07/$08.00+0     doi:10.1128/MCB.02366-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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