This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gordon, M.
Right arrow Articles by Cairns, B. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gordon, M.
Right arrow Articles by Cairns, B. R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2007, p. 4058-4069, Vol. 27, No. 11
0270-7306/07/$08.00+0     doi:10.1128/MCB.02044-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Genome-Wide Dynamics of SAPHIRE, an Essential Complex for Gene Activation and Chromatin Boundaries{triangledown}

Matthew Gordon,1 Derick G. Holt,1 Anil Panigrahi,1 Brian T. Wilhelm,2 Hediye Erdjument-Bromage,3 Paul Tempst,3 Jürg Bähler,2 and Bradley R. Cairns1*

Howard Hughes Medical Institute, Department of Oncological Sciences, Huntsman Cancer Institute, 2000 Circle of Hope, University of Utah, Salt Lake City, Utah 84112,1 The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, United Kingdom,2 Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 100213

Received 1 November 2006/ Returned for modification 19 December 2006/ Accepted 9 March 2007

In this study, we characterize a four-protein nucleosome-binding complex from Schizosaccharomyces pombe, termed SAPHIRE, that includes two orthologs of human Lsd1, a histone demethylase. The SAPHIRE complex is essential for cell viability, whereas saphire mutants lacking key conserved catalytic residues are viable but thermosensitive, suggesting that SAPHIRE has both an important enzymatic function and an essential nonenzymatic function. SAPHIRE is present in (or adjacent to) particular heterochromatic loci and also in the transcription start site regions of many highly active polymerase II genes. However, ribosomal protein genes are notably SAPHIRE deficient. SAPHIRE promotes activation, as target genes are selectively attenuated in saphire mutants. Interestingly, saphire mutants display increased histone H3 lysine 4 dimethylation, a modification typically associated with euchromatin. SAPHIRE localization is dynamic, as activated genes rapidly acquire SAPHIRE. Furthermore, saphire mutants dramatically shift a heterochromatin-euchromatin boundary in Chr1, suggesting a novel role in boundary regulation.

* Corresponding author. Mailing address: University of Utah, Huntsman Cancer Institute, 2000 Circle of Hope, Salt Lake City, UT 84112. Phone: (801) 585-1822. Fax: (801) 585-6410. E-mail: bcairns{at}hci.utah.edu

{triangledown} Published ahead of print on 19 March 2007.

Molecular and Cellular Biology, June 2007, p. 4058-4069, Vol. 27, No. 11
0270-7306/07/$08.00+0     doi:10.1128/MCB.02044-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nottke, A., Colaiacovo, M. P., Shi, Y. (2009). Developmental roles of the histone lysine demethylases. Development 136: 879-889 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»