This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luke-Glaser, S.
Right arrow Articles by Pintard, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luke-Glaser, S.
Right arrow Articles by Pintard, L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2007, p. 4526-4540, Vol. 27, No. 12
0270-7306/07/$08.00+0     doi:10.1128/MCB.01724-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

CIF-1, a Shared Subunit of the COP9/Signalosome and Eukaryotic Initiation Factor 3 Complexes, Regulates MEL-26 Levels in the Caenorhabditis elegans Embryo{triangledown}

Sarah Luke-Glaser,1 Marcia Roy,2 Brett Larsen,2 Thierry Le Bihan,3 Pavel Metalnikov,2 Mike Tyers,2,{dagger} Matthias Peter,1,{dagger} and Lionel Pintard2,4*,{dagger}

Swiss Federal Institute of Technology Zurich (ETH), Institute of Biochemistry, HPM G8, ETH Hoenggerberg, 8093 Zurich, Switzerland,1 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada,2 The Campbell Family Institute for Breast Cancer Research, Ontario Cancer Institute, Toronto, Ontario M5G 2M9, Canada,3 Institut Jacques Monod-CNRS, Université Paris VI et VII, 2 Place Jussieu Tour 43, 75251 Paris Cedex 05, France4

Received 12 September 2006/ Returned for modification 16 November 2006/ Accepted 7 March 2007

The COP9/signalosome (CSN) is an evolutionarily conserved macromolecular complex that regulates the cullin-RING ligase (CRL) class of E3 ubiquitin ligases, primarily by removing the ubiquitin-like protein Nedd8 from the cullin subunit. In the Caenorhabditis elegans embryo, the CSN controls the degradation of the microtubule-severing protein MEI-1 through CUL-3 deneddylation. However, the molecular mechanisms of CSN function and its subunit composition remain to be elucidated. Here, using a proteomic approach, we have characterized the CSN and CUL-3 complexes from C. elegans embryos. We show that the CSN physically interacts with the CUL-3-based CRL and regulates its activity by counteracting the autocatalytic instability of the substrate-specific adaptor MEL-26. Importantly, we identified the uncharacterized protein K08F11.3/CIF-1 (for CSN-eukaryotic initiation factor 3 [eIF3]) as a stoichiometric and functionally important subunit of the CSN complex. CIF-1 appears to be the only ortholog of Csn7 encoded by the C. elegans genome, but it also exhibits extensive sequence similarity to eIF3m family members, which are required for the initiation of protein translation. Indeed, CIF-1 binds eIF-3.F and inactivation of cif-1 impairs translation in vivo. Taken together, our results indicate that CIF-1 is a shared subunit of the CSN and eIF3 complexes and may therefore link protein translation and degradation.

* Corresponding author. Mailing address: Institut Jacques Monod-Centre National de la Recherche Scientifique, Université 6 et 7, 2, place Jussieu Tour 43, 75251 Paris Cedex 05, France. Phone: (33)-1-44-27-77-98. Fax: (33)-1-44-27-52-65. E-mail: pintard.lionel{at}ijm.jussieu.fr

{triangledown} Published ahead of print on 2 April 2007.

{dagger} Mike Tyers, Matthias Peter, and Lionel Pintard contributed equally to this study.

Molecular and Cellular Biology, June 2007, p. 4526-4540, Vol. 27, No. 12
0270-7306/07/$08.00+0     doi:10.1128/MCB.01724-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kato, J.-y., Yoneda-Kato, N. (2009). Mammalian COP9 signalosome. GENES CELLS 14: 1209-1225 [Abstract] [Full Text]  
  • Li, W., DeBella, L. R., Guven-Ozkan, T., Lin, R., Rose, L. S. (2009). An eIF4E-binding protein regulates katanin protein levels in C. elegans embryos. JCB 187: 33-42 [Abstract] [Full Text]  
  • Miller, R. K., Qadota, H., Stark, T. J., Mercer, K. B., Wortham, T. S., Anyanful, A., Benian, G. M. (2009). CSN-5, a Component of the COP9 Signalosome Complex, Regulates the Levels of UNC-96 and UNC-98, Two Components of M-lines in Caenorhabditis elegans Muscle. Mol. Biol. Cell 20: 3608-3616 [Abstract] [Full Text]  
  • Olma, M. H., Roy, M., Le Bihan, T., Sumara, I., Maerki, S., Larsen, B., Quadroni, M., Peter, M., Tyers, M., Pintard, L. (2009). An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J. Cell Sci. 122: 1035-1044 [Abstract] [Full Text]  
  • Stuttmann, J., Lechner, E., Guerois, R., Parker, J. E., Nussaume, L., Genschik, P., Noel, L. D. (2009). COP9 Signalosome- and 26S Proteasome-dependent Regulation of SCFTIR1 Accumulation in Arabidopsis. J. Biol. Chem. 284: 7920-7930 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»