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Molecular and Cellular Biology, July 2007, p. 4796-4806, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.00305-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Boundary Element-Associated Factor 32B Connects Chromatin Domains to the Nuclear Matrix{triangledown}

Rashmi U. Pathak,1 Nandini Rangaraj,1 Satish Kallappagoudar,1 Krishnaveni Mishra,2* and Rakesh K. Mishra1*

Center for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India,1 Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India2

Received 20 February 2007/ Returned for modification 14 March 2007/ Accepted 24 April 2007

Chromatin domain boundary elements demarcate independently regulated domains of eukaryotic genomes. While a few such boundary sequences have been studied in detail, only a small number of proteins that interact with them have been identified. One such protein is the boundary element-associated factor (BEAF), which binds to the scs' boundary element of Drosophila melanogaster. It is not clear, however, how boundary elements function. In this report we show that BEAF is associated with the nuclear matrix and map the domain required for matrix association to the middle region of the protein. This region contains a predicted coiled-coil domain with several potential sites for posttranslational modification. We demonstrate that the DNA sequences that bind to BEAF in vivo are also associated with the nuclear matrix and colocalize with BEAF. These results suggest that boundary elements may function by tethering chromatin to nuclear architectural components and thereby provide a structural basis for compartmentalization of the genome into functionally independent domains.

* Corresponding author. Mailing address for Krishnaveni Mishra: Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, India. Phone: 91 40 23134544. Fax: 91 40 23010120. E-mail: kmsl{at}uohyd.ernet.in. Mailing address for Rakesh K. Mishra: Center for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India. Phone: 91 40 271 92658. Fax: 91 40 271 60591. E-mail: mishra{at}ccmb.res.in

{triangledown} Published ahead of print on 7 May 2007.

Molecular and Cellular Biology, July 2007, p. 4796-4806, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.00305-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gurudatta, B. V., Corces, V. G. (2009). Chromatin insulators: lessons from the fly. Brief Funct Genomic Proteomic 8: 276-282 [Abstract] [Full Text]  
  • Jiang, N., Emberly, E., Cuvier, O., Hart, C. M. (2009). Genome-Wide Mapping of Boundary Element-Associated Factor (BEAF) Binding Sites in Drosophila melanogaster Links BEAF to Transcription. Mol. Cell. Biol. 29: 3556-3568 [Abstract] [Full Text]  


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