This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dong, X.
Right arrow Articles by Lye, S. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dong, X.
Right arrow Articles by Lye, S. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, July 2007, p. 4863-4875, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.02144-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Transcriptional Activity of Androgen Receptor Is Modulated by Two RNA Splicing Factors, PSF and p54nrb{triangledown}

Xuesen Dong,1 Joan Sweet,2 John R. G. Challis,3 Theodore Brown,1 and Stephen J. Lye1*

Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Canada M5G 1X5,1 University Health Network, University of Toronto, 200 Elizabeth Street, Toronto, Canada M5G 2M9,2 Department of Physiology, University of Toronto, Medical Sciences Building, 1 King's College Circle, Toronto, Canada M5S 1A83

Received 15 November 2006/ Returned for modification 16 January 2007/ Accepted 11 April 2007

Nuclear receptors regulate gene activation or repression through dynamic interactions with coregulators. The interactions between nuclear receptors and RNA splicing factors link gene transcription initiation with pre-mRNA splicing, providing a coordinated control of the products of gene transcription. Here we report that two RNA splicing factors, PTB-associated splicing factor (PSF) and p54nrb, synergistically form protein complexes with the androgen receptor (AR) in a ligand-independent manner and inhibit its transcriptional activity. PSF does not affect AR protein stability, as in the case of the progesterone receptor, but impedes the interaction of AR with the androgen response element. Both splicing factors interact directly with mSin3A and attract mSin3A to the AR complex in a synergistic manner. The suppression of AR transcriptional activity by PSF and p54nrb is reversed by the inhibition of histone deacetylase activity. These data demonstrated that PSF and p54nrb complex with AR and play a key role in modulating AR-mediated gene transcription.

* Corresponding author. Mailing address: Samuel Lunenfeld Research Institute, Mount Sinai Hospital, University of Toronto, 600 University Avenue, Toronto, Canada M5G 1X5. Phone: (416) 586-4800, ext. 8640. Fax: (416) 586-8857. E-mail: lye{at}mshri.on.ca

{triangledown} Published ahead of print on 23 April 2007.

Molecular and Cellular Biology, July 2007, p. 4863-4875, Vol. 27, No. 13
0270-7306/07/$08.00+0     doi:10.1128/MCB.02144-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Li, S., Kuhne, W. W., Kulharya, A., Hudson, F. Z., Ha, K., Cao, Z., Dynan, W. S. (2009). Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance. Nucleic Acids Res 37: 6746-6753 [Abstract] [Full Text]  
  • Yu, Z., Wang, A. M., Robins, D. M., Lieberman, A. P. (2009). Altered RNA splicing contributes to skeletal muscle pathology in Kennedy disease knock-in mice. DMM 2: 500-507 [Abstract] [Full Text]  
  • Dong, X., Yu, C., Shynlova, O., Challis, J. R. G., Rennie, P. S., Lye, S. J. (2009). p54nrb Is a Transcriptional Corepressor of the Progesterone Receptor that Modulates Transcription of the Labor-Associated Gene, Connexin 43 (Gja1). Mol. Endocrinol. 23: 1147-1160 [Abstract] [Full Text]  
  • Tyson-Capper, A J, Shiells, E A, Robson, S C (2009). Interplay between polypyrimidine tract binding protein-associated splicing factor and human myometrial progesterone receptors. J Mol Endocrinol 43: 29-41 [Abstract] [Full Text]  
  • Burton, T. R., Eisenstat, D. D., Gibson, S. B. (2009). BNIP3 (Bcl-2 19 kDa Interacting Protein) Acts as Transcriptional Repressor of Apoptosis-Inducing Factor Expression Preventing Cell Death in Human Malignant Gliomas. J. Neurosci. 29: 4189-4199 [Abstract] [Full Text]  
  • Jacobs, F. M. J., van Erp, S., van der Linden, A. J. A., von Oerthel, L., Burbach, J. P. H., Smidt, M. P. (2009). Pitx3 potentiates Nurr1 in dopamine neuron terminal differentiation through release of SMRT-mediated repression. Development 136: 531-540 [Abstract] [Full Text]  
  • Zhong, N., Xu, J. (2008). Synergistic activation of the human MnSOD promoter by DJ-1 and PGC-1{alpha}: regulation by SUMOylation and oxidation. Hum Mol Genet 17: 3357-3367 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»