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Molecular and Cellular Biology, August 2007, p. 5306-5315, Vol. 27, No. 15
0270-7306/07/$08.00+0     doi:10.1128/MCB.01912-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Transcription Corepressor LEUNIG Interacts with the Histone Deacetylase HDA19 and Mediator Components MED14 (SWP) and CDK8 (HEN3) To Repress Transcription{triangledown} ,{dagger}

Deyarina Gonzalez, Adam J. Bowen, Thomas S. Carroll, and R. Steven Conlan*

Institute of Life Science, School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom

Received 10 October 2006/ Returned for modification 8 January 2007/ Accepted 17 May 2007

Transcription corepressors are general regulators controlling the expression of genes involved in multiple signaling pathways and developmental programs. Repression is mediated through mechanisms including the stabilization of a repressive chromatin structure over control regions and regulation of Mediator function inhibiting RNA polymerase II activity. Using whole-genome arrays we show that the Arabidopsis thaliana corepressor LEUNIG, a member of the GroTLE transcription corepressor family, regulates the expression of multiple targets in vivo. LEUNIG has a role in the regulation of genes involved in a number of different physiological processes including disease resistance, DNA damage response, and cell signaling. We demonstrate that repression of in vivo LEUNIG targets is achieved through histone deacetylase (HDAC)-dependent and -independent mechanisms. HDAC-dependent mechanisms involve direct interaction with HDA19, a class 1 HDAC, whereas an HDAC-independent repression activity involves interactions with the putative Arabidopsis Mediator components AtMED14/SWP and AtCDK8/HEN3. We suggest that changes in chromatin structure coupled with regulation of Mediator function are likely to be utilized by LEUNIG in the repression of gene transcription.

* Corresponding author. Mailing address: Institute of Life Science, School of Medicine, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, United Kingdom. Phone: 441792295386. Fax: 441792602280. E-mail: r.s.conlan{at}swansea.ac.uk

{triangledown} Published ahead of print on 25 May 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, August 2007, p. 5306-5315, Vol. 27, No. 15
0270-7306/07/$08.00+0     doi:10.1128/MCB.01912-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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