This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Menoni, H.
Right arrow Articles by Angelov, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Menoni, H.
Right arrow Articles by Angelov, D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2007, p. 5949-5956, Vol. 27, No. 17
0270-7306/07/$08.00+0     doi:10.1128/MCB.00376-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

ATP-Dependent Chromatin Remodeling Is Required for Base Excision Repair in Conventional but Not in Variant H2A.Bbd Nucleosomes{triangledown}

Hervé Menoni,1,2,3 Didier Gasparutto,4 Ali Hamiche,5 Jean Cadet,4 Stefan Dimitrov,6 Philippe Bouvet,1,2 and Dimitar Angelov1,2*

Laboratoire Joliot-Curie, CNRS-USR3010, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 7, France,1 Laboratoire de Biologie Moléculaire de la Cellule, CNRS-UMR5239/INRA-1237/IFR128 Biosciences, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 7, France,2 Institute of Solid State Physics, Bulgarian Academy of Sciences, 1784 Sofia, Bulgaria,3 Laboratoire des Lésions des Acides Nucléiques, Département de Recherche Fondamentale sur la Matière Condensée, Service de Chimie Inorganique et Biologique, UMR E3 CEA-UJF, CEA-Grenoble, F-38054 Grenoble Cedex 9, France,4 Institut André Lwoff, CNRS UPR 9079, Villejuif, France,5 CRI INSERM U823 Université Joseph Fourier, Institut Albert Bonniot, Site Santé La Tronche, BP170, 38042 Grenoble Cedex 9, France6

Received 1 March 2007/ Returned for modification 1 April 2007/ Accepted 11 June 2007

In eukaryotes, base excision repair (BER) is responsible for the repair of oxidatively generated lesions. The mechanism of BER on naked DNA substrates has been studied in detail, but how it operates on chromatin remains unclear. Here we have studied the mechanism of BER by introducing a single 8-oxo-7,8-dihydroguanine (8-oxoG) lesion in the DNA of reconstituted positioned conventional and histone variant H2A.Bbd nucleosomes. We found that 8-oxoguanine DNA glycosylase, apurinic/apyrimidinic endonuclease, and polymerase ß activities were strongly reduced in both types of nucleosomes. In conventional nucleosomes SWI/SNF stimulated the processing of 8-oxoG by each one of the three BER repair factors to efficiencies similar to those for naked DNA. Interestingly, SWI/SNF-induced remodeling, but not mobilization of conventional nucleosomes, was required to achieve this effect. A very weak effect of SWI/SNF on the 8-oxoG BER removal in H2A.Bbd histone variant nucleosomes was observed. The possible implications of our data for the understanding of in vivo mechanisms of BER are discussed.

* Corresponding author. Mailing address: Laboratoire Joliot-Curie, CNRS-USR3010, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 7, France. Phone: 33472728898. Fax: 33472728016. E-mail: Dimitar.Anguelov{at}ens-lyon.fr

{triangledown} Published ahead of print on 25 June 2007.

Molecular and Cellular Biology, September 2007, p. 5949-5956, Vol. 27, No. 17
0270-7306/07/$08.00+0     doi:10.1128/MCB.00376-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ishibashi, T., So, K., Cupples, C. G., Ausio, J. (2008). MBD4-Mediated Glycosylase Activity on a Chromatin Template Is Enhanced by Acetylation. Mol. Cell. Biol. 28: 4734-4744 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»