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Molecular and Cellular Biology, October 2007, p. 6782-6793, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.01097-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Dynamic Scaffold of Pre-snoRNP Factors Facilitates Human Box C/D snoRNP Assembly{triangledown}

Kenneth Scott McKeegan,1 Charles Maurice Debieux,1 Séverine Boulon,2 Edouard Bertrand,2 and Nicholas James Watkins1*

ICaMB, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom,1 IGMM, CNRS UMR5535, Montpellier, France2

Received 20 June 2007/ Accepted 6 July 2007

The box C/D small nucleolar RNPs (snoRNPs) are essential for the processing and modification of rRNA. The core box C/D proteins are restructured during human U3 box C/D snoRNP biogenesis; however, the molecular basis of this is unclear. Here we show that the U8 snoRNP is also restructured, suggesting that this may occur with all box C/D snoRNPs. We have characterized four novel human biogenesis factors (BCD1, NOP17, NUFIP, and TAF9) which, along with the ATPases TIP48 and TIP49, are likely to be involved in the formation of the pre-snoRNP. We have analyzed the in vitro protein-protein interactions between the assembly factors and core box C/D proteins. Surprisingly, this revealed few interactions between the individual core box C/D proteins. However, the novel biogenesis factors and TIP48 and TIP49 interacted with one or more of the core box C/D proteins, implying that they mediate the assembly of the pre-snoRNP. Consistent with this, we show that NUFIP bridges interactions between the core box C/D proteins in a partially reconstituted pre-snoRNP. Restructuring of the core complex probably reflects the conversion of the pre-snoRNP, where core protein-protein interactions are maintained by the bridging biogenesis factors, to the mature snoRNP.

* Corresponding author. Mailing address: Institute for Cell and Molecular Biosciences, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 44 191 222 6991. Fax: 44 191 222 7424. E-mail: n.j.watkins{at}ncl.ac.uk

{triangledown} Published ahead of print on 16 July 2007.

Molecular and Cellular Biology, October 2007, p. 6782-6793, Vol. 27, No. 19
0270-7306/07/$08.00+0     doi:10.1128/MCB.01097-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McKeegan, K. S., Debieux, C. M., Watkins, N. J. (2009). Evidence that the AAA+ Proteins TIP48 and TIP49 Bridge Interactions between 15.5K and the Related NOP56 and NOP58 Proteins during Box C/D snoRNP Biogenesis. Mol. Cell. Biol. 29: 4971-4981 [Abstract] [Full Text]  
  • Renvoise, B., Colasse, S., Burlet, P., Viollet, L., Meier, U. T., Lefebvre, S. (2009). The loss of the snoRNP chaperone Nopp140 from Cajal bodies of patient fibroblasts correlates with the severity of spinal muscular atrophy. Hum Mol Genet 18: 1181-1189 [Abstract] [Full Text]  
  • Ducat, D., Kawaguchi, S.-i., Liu, H., Yates, J. R. III, Zheng, Y. (2008). Regulation of Microtubule Assembly and Organization in Mitosis by the AAA+ ATPase Pontin. Mol. Biol. Cell 19: 3097-3110 [Abstract] [Full Text]  
  • Watkins, N. J., Lemm, I., Luhrmann, R. (2007). Involvement of Nuclear Import and Export Factors in U8 Box C/D snoRNP Biogenesis. Mol. Cell. Biol. 27: 7018-7027 [Abstract] [Full Text]  


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