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Molecular and Cellular Biology, November 2007, p. 7641-7648, Vol. 27, No. 21
0270-7306/07/$08.00+0     doi:10.1128/MCB.00742-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Regulation of Gene Transcription by the Histone H2A N-Terminal Domain{triangledown} ,{dagger}

Michael A. Parra and John J. Wyrick*

School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660

Received 27 April 2007/ Returned for modification 19 June 2007/ Accepted 13 August 2007

Histone N-terminal domains play critical roles in regulating chromatin structure and gene transcription. Relatively little is known, however, about the role of the histone H2A N-terminal domain in transcription regulation. We have used DNA microarrays to characterize the changes in genome-wide expression caused by mutations in the N-terminal domain of histone H2A. Our results indicate that the N-terminal domain of histone H2A functions primarily to repress the transcription of a large subset of the Saccharomyces cerevisiae genome and that most of the H2A-repressed genes are also repressed by the histone H2B N-terminal domain. Using the histone H2A microarray data, we selected three reporter genes (BNA1, BNA2, and GCY1), which we subsequently used to map regions in the H2A N-terminal domain responsible for this transcriptional repression. These studies revealed that a small subdomain in the H2A N-terminal tail, comprised of residues 16 to 20, is required for the transcriptional repression of these reporter genes. Deletion of either the entire histone H2A N-terminal domain or just this small subdomain imparts sensitivity to UV irradiation. Finally, we show that two residues in this H2A subdomain, serine-17 and arginine-18, are specifically required for the transcriptional repression of the BNA2 reporter gene.

* Corresponding author. Mailing address: School of Molecular Biosciences, Washington State University, Fulmer Hall 675, Pullman, WA 99164-4660. Phone: (509) 335-8785. Fax: (509) 335-9688. E-mail: jwyrick{at}wsu.edu

{triangledown} Published ahead of print on 27 August 2007.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, November 2007, p. 7641-7648, Vol. 27, No. 21
0270-7306/07/$08.00+0     doi:10.1128/MCB.00742-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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