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Molecular and Cellular Biology, December 2007, p. 8364-8373, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01253-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Activation of the G2/M-Specific Gene CLB2 Requires Multiple Cell Cycle Signals{triangledown}

J. Veis,{dagger} H. Klug,{dagger} M. Koranda,{ddagger} and G. Ammerer*

Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Biochemistry, University of Vienna, Dr. Bohrgasse 9, 1030 Vienna, Austria

Received 12 July 2007/ Returned for modification 12 August 2007/ Accepted 15 September 2007

In budding yeast (Saccharomyces cerevisiae), the periodic expression of the G2/M-specific gene CLB2 depends on a DNA binding complex that mediates its repression during G1 and activation from the S phase to the exit of mitosis. The switch from low to high expression levels depends on the transcriptional activator Ndd1. We show that the inactivation of the Sin3 histone deacetylase complex bypasses the essential role of Ndd1 in cell cycle progression. Sin3 and its catalytic subunit Rpd3 associate with the CLB2 promoter during the G1 phase of the cell cycle. Both proteins dissociate from the promoter at the onset of the S phase and reassociate during G2 phase. Sin3 removal coincides with a transient increase in histone H4 acetylation followed by the expulsion of at least one nucleosome from the promoter region. Whereas the first step depends on Cdc28/Cln1 activity, Ndd1 function is required for the second step. Since the removal of Sin3 is independent of Ndd1 recruitment and Cdc28/Clb activity it represents a unique regulatory step which is distinct from transcriptional activation.

* Corresponding author. Mailing address: Department of Biochemistry, University of Vienna, Dr. Bohrgasse 9, 1030 Vienna, Austria. Phone: 43 1 4277 52816. Fax: 43 1 4277 9 528. E-mail: gustav.ammerer{at}univie.ac.at

{triangledown} Published ahead of print on 1 October 2007.

{dagger} J.V. and H.K. contributed equally to the work.

{ddagger} Present address: Institute of Molecular Pathology, Vienna, Austria, Dr. Bohrgasse 7, 1030 Vienna, Austria.

Molecular and Cellular Biology, December 2007, p. 8364-8373, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01253-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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