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Molecular and Cellular Biology, December 2007, p. 8409-8418, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01543-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Requirement of Nse1, a Subunit of the Smc5-Smc6 Complex, for Rad52-Dependent Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae{triangledown}

Sergio R. Santa Maria, Venkateswarlu Gangavarapu, Robert E. Johnson, Louise Prakash, and Satya Prakash*

Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas 77555-1061

Received 23 August 2007/ Returned for modification 14 September 2007/ Accepted 20 September 2007

In Saccharomyces cerevisiae, postreplication repair (PRR) of UV-damaged DNA occurs by a Rad6-Rad18- and an Mms2-Ubc13-Rad5-dependent pathway or by a Rad52-dependent pathway. The Rad5 DNA helicase activity is specialized for promoting replication fork regression and template switching; previously, we suggested a role for the Rad5-dependent PRR pathway when the lesion is located on the leading strand and a role for the Rad52 pathway when the lesion is located on the lagging strand. In this study, we present evidence for the requirement of Nse1, a subunit of the Smc5-Smc6 complex, in Rad52-dependent PRR, and our genetic analyses suggest a role for the Nse1 and Mms21 E3 ligase activities associated with this complex in this repair mode. We discuss the possible ways by which the Smc5-Smc6 complex, including its associated ubiquitin ligase and SUMO ligase activities, might contribute to the Rad52-dependent nonrecombinational and recombinational modes of PRR.

* Corresponding author. Mailing address: University of Texas Medical Branch at Galveston, 301 University Blvd., Galveston, TX 77555-1061. Phone: (409) 747-8602. Fax: (409) 747-8608. E-mail: s.prakash{at}utmb.edu

{triangledown} Published ahead of print on 4 September 2007.

Molecular and Cellular Biology, December 2007, p. 8409-8418, Vol. 27, No. 23
0270-7306/07/$08.00+0     doi:10.1128/MCB.01543-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sollier, J., Driscoll, R., Castellucci, F., Foiani, M., Jackson, S. P., Branzei, D. (2009). The Saccharomyces cerevisiae Esc2 and Smc5-6 Proteins Promote Sister Chromatid Junction-mediated Intra-S Repair. Mol. Biol. Cell 20: 1671-1682 [Abstract] [Full Text]  


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