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Molecular and Cellular Biology, February 2007, p. 1467-1485, Vol. 27, No. 4
0270-7306/07/$08.00+0     doi:10.1128/MCB.01360-06

STAMP, a Novel Predicted Factor Assisting TIF2 Actions in Glucocorticoid Receptor-Mediated Induction and Repression{triangledown}

Yuanzheng He and S. Stoney Simons Jr.*

Steroid Hormones Section, Clinical Endocrinology Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland

Received 25 July 2006/ Returned for modification 1 September 2006/ Accepted 8 November 2006

The coactivator TIF2 was predicted to interact with an unknown factor to modify both the relative inhibition in glucocorticoid receptor (GR)-mediated gene repression and several parameters of agonists and antisteroids in GR-regulated induction. Here, we describe the isolation and characterization of the predicted factor as a new 1,277-amino-acid endogenous protein (STAMP). STAMP associates with coactivators (TIF2 and SRC-1) and is selective for a subset of the steroid/nuclear receptors including GRs. Transfected STAMP increases the effects of TIF2 in GR-mediated repression and induction. Conversely, the levels of both induction and repression of endogenous genes are reduced when STAMP small interfering RNAs are used to lower the level of endogenous STAMP. Endogenous STAMP colocalizes with GR in intact cells and is recruited to the promoters of endogenous GR-induced and -repressed genes. We suggest that STAMP is an important new, downstream component of GR action in both gene activation and gene repression.

* Corresponding author. Mailing address: National Institute of Diabetes and Digestive and Kidney Diseases, Clinical Endocrinology Branch, National Institutes of Health, Bldg. 10, Room 8N307B, Bethesda, MD 20892-1772. Phone: (301) 496-6796. Fax: (301) 402-3572. E-mail: steroids{at}helix.nih.gov.

{triangledown} Published ahead of print on 20 November 2006.

Molecular and Cellular Biology, February 2007, p. 1467-1485, Vol. 27, No. 4
0270-7306/07/$08.00+0     doi:10.1128/MCB.01360-06






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