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Molecular and Cellular Biology, March 2007, p. 2215-2228, Vol. 27, No. 6
0270-7306/07/$08.00+0     doi:10.1128/MCB.01454-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Colitis and Colitis-Associated Cancer Are Exacerbated in Mice Deficient for Tumor Protein 53-Induced Nuclear Protein 1{triangledown}

Julien Gommeaux,1,2,{dagger} Carla Cano,1,2,{dagger} Stéphane Garcia,1,2 Meritxell Gironella,1,2 Sylvia Pietri,3 Marcel Culcasi,3 Marie-Josèphe Pébusque,1,2 Bernard Malissen,4 Nelson Dusetti,1,2 Juan Iovanna,1,2 and Alice Carrier1,2*

INSERM, U624 Stress cellulaire, F-13288 Marseille, France,1 Aix-Marseille Université, Campus de Luminy, F-13000 Marseille, France,2 Aix-Marseille Université, Faculté des Sciences de Saint-Jérôme, SREP-Sondes Moléculaires en Biologie, CNRS UMR6517, F-13397 Marseille, France,3 Aix-Marseille Université, Centre d'Immunologie de Marsaille-Luminy, F-13288 Marseille, France4

Received 7 August 2006/ Returned for modification 7 September 2006/ Accepted 19 December 2006

Tumor protein 53-induced nuclear protein 1 (TP53INP1) is an antiproliferative and proapoptotic protein involved in cell stress response. To address its physiological roles in colorectal cancer and colitis, we generated and tested the susceptibility of Trp53inp1-deficient mice to the development of colorectal tumors induced by injection of the carcinogen azoxymethane followed by dextran sulfate sodium (DSS)-induced chronic colitis. Trp53inp1-deficient mice showed an increased incidence and multiplicity of tumors compared to those of wild-type (WT) mice. Furthermore, acute colitis induced by DSS treatment was more severe in Trp53inp1-deficient mice than in WT mice. Treatment with the antioxidant N-acetylcysteine prevented colitis and colitis-associated tumorigenesis more efficiently in WT mice than in Trp53inp1-deficient mice, suggesting a higher oxidative load in the latter. Consistently, we demonstrated by electron spin resonance and spin trapping that colons derived from deficient mice produced more free radicals than those of the WT during colitis and that the basal blood level of the antioxidant ascorbate was decreased in Trp53inp1-deficient mice. Collectively, these results indicate that the oxidative load is higher in Trp53inp1-deficient mice than in WT mice, generating a more-severe DSS-induced colitis, which favors development of colorectal tumors in Trp53inp1-deficient mice. Therefore, TP53INP1 is a potential target for the prevention of colorectal cancer in patients with inflammatory bowel disease.

* Corresponding author. Mailing address: INSERM, U624 Stress cellulaire, Case 915 Parc Scientifique de Luminy, 13288 Marseille Cedex 9, France. Phone: 33 4 91 82 75 53. Fax: 33 4 91 82 60 83. E-mail: carrier{at}marseille.inserm.fr.

{triangledown} Published ahead of print on 22 January 2007.

{dagger} These authors contributed equally to this work.

Molecular and Cellular Biology, March 2007, p. 2215-2228, Vol. 27, No. 6
0270-7306/07/$08.00+0     doi:10.1128/MCB.01454-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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