This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherriff, J. A.
Right arrow Articles by Mellor, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherriff, J. A.
Right arrow Articles by Mellor, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2007, p. 2848-2860, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01798-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The Isw2 Chromatin-Remodeling ATPase Cooperates with the Fkh2 Transcription Factor To Repress Transcription of the B-Type Cyclin Gene CLB2{triangledown}

Julia A. Sherriff, Nicholas A. Kent, and Jane Mellor*

Division of Molecular Genetics, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom

Received 22 September 2006/ Returned for modification 24 October 2006/ Accepted 24 January 2007

Forkhead (Fkh) transcription factors influence cell death, proliferation, and differentiation and the cell cycle. In Saccharomyces cerevisiae, Fkh2 both activates and represses transcription of CLB2, encoding a B-type cyclin. CLB2 is expressed during G2/M phase and repressed during G1. Fkh2 recruits the coactivator Ndd1, an interaction which is promoted by Clb2/Cdk1-dependent phosphorylation of Ndd1, suggesting that CLB2 is autoregulated. Ndd1 is proposed to function by antagonizing Fkh2-mediated repression, but nothing is known about the mechanism. Here we ask how Fkh2 represses CLB2. We show that Fkh2 controls a repressive chromatin structure that initiates in the early coding region of CLB2 and spreads up the promoter during the M and G1 phases. The Isw2 chromatin-remodeling ATPase cooperates with Fkh2 to remodel the chromatin and repress CLB2 expression throughout the cell cycle. In addition, the related factors Isw1 and Fkh1 configure the chromatin at the early coding region and negatively regulate CLB2 expression but only during G2/M phase. Thus, the cooperative actions of two forkhead transcription factors and two chromatin-remodeling ATPases combine to regulate CLB2. We propose that chromatin-mediated repression by Isw1 and Isw2 may serve to limit activation of CLB2 expression by the Clb2/Cdk1 kinase during G2/M and to fully repress expression during G1.

* Corresponding author. Mailing address: Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom. Phone: 44 1 865 275303. Fax: 44 1 865 275297. E-mail: jane.mellor{at}bioch.ox.ac.uk

{triangledown} Published ahead of print on 5 February 2007.

Molecular and Cellular Biology, April 2007, p. 2848-2860, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.01798-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pondugula, S., Neef, D. W., Voth, W. P., Darst, R. P., Dhasarathy, A., Reynolds, M. M., Takahata, S., Stillman, D. J., Kladde, M. P. (2009). Coupling Phosphate Homeostasis to Cell Cycle-Specific Transcription: Mitotic Activation of Saccharomyces cerevisiae PHO5 by Mcm1 and Forkhead Proteins. Mol. Cell. Biol. 29: 4891-4905 [Abstract] [Full Text]  
  • Pinskaya, M., Nair, A., Clynes, D., Morillon, A., Mellor, J. (2009). Nucleosome Remodeling and Transcriptional Repression Are Distinct Functions of Isw1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 29: 2419-2430 [Abstract] [Full Text]  
  • Frydlova, I., Malcova, I., Vasicova, P., Hasek, J. (2009). Deregulation of DSE1 Gene Expression Results in Aberrant Budding within the Birth Scar and Cell Wall Integrity Pathway Activation in Saccharomyces cerevisiae. Eukaryot Cell 8: 586-594 [Abstract] [Full Text]  
  • Casey, L., Patterson, E. E., Muller, U., Fox, C. A. (2008). Conversion of a Replication Origin to a Silencer through a Pathway Shared by a Forkhead Transcription Factor and an S Phase Cyclin. Mol. Biol. Cell 19: 608-622 [Abstract] [Full Text]  
  • Veis, J., Klug, H., Koranda, M., Ammerer, G. (2007). Activation of the G2/M-Specific Gene CLB2 Requires Multiple Cell Cycle Signals. Mol. Cell. Biol. 27: 8364-8373 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»