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Molecular and Cellular Biology, April 2007, p. 2870-2885, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.02323-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Modular Enhancer Is Differentially Regulated by GATA and NFAT Elements That Direct Different Tissue-Specific Patterns of Nucleosome Positioning and Inducible Chromatin Remodeling{triangledown}

Andrew G. Bert,1,{dagger} Brett V. Johnson,2,{dagger},{ddagger} Euan W. Baxter,2 and Peter N. Cockerill2*

Hanson Institute, Institute of Medical and Veterinary Science, Adelaide 5000, Australia,1 Experimental Haematology, Leeds Institute of Molecular Medicine, St. James's University Hospital, Leeds LS9 7TF, United Kingdom2

Received 12 December 2006/ Returned for modification 24 January 2007/ Accepted 27 January 2007

We investigated alternate mechanisms employed by enhancers to position and remodel nucleosomes and activate tissue-specific genes in divergent cell types. We demonstrated that the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene enhancer is modular and recruits different sets of transcription factors in T cells and myeloid cells. The enhancer recruited distinct inducible tissue-specific enhanceosome-like complexes and directed nucleosomes to different positions in these cell types. In undifferentiated T cells, the enhancer was activated by inducible binding of two NFAT/AP-1 complexes which disrupted two specifically positioned nucleosomes (N1 and N2). In myeloid cells, the enhancer was remodeled by GATA factors which constitutively displaced an upstream nucleosome (N0) and cooperated with inducible AP-1 elements to activate transcription. In mast cells, which express both GATA-2 and NFAT, these two pathways combined to activate the enhancer and generate high-level gene expression. At least 5 kb of the GM-CSF locus was organized as an array of nucleosomes with fixed positions, but the enhancer adopted different nucleosome positions in T cells and mast cells. Furthermore, nucleosomes located between the enhancer and promoter were mobilized upon activation in an enhancer-dependent manner. These studies reveal that distinct tissue-specific mechanisms can be used either alternately or in combination to activate the same enhancer.

* Corresponding author. Mailing address: Experimental Haematology, Leeds Institute of Molecular Medicine, Wellcome Trust Brenner Building, St. James's University Hospital, Leeds LS9 7TF, United Kingdom. Phone: 44 113 3438639. Fax: 44 113 3438502. E-mail: p.n.cockerill{at}leeds.ac.uk

{triangledown} Published ahead of print on 5 February 2007.

{dagger} A.G.B. and B.V.J. contributed equally to this study.

{ddagger} Present address: School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide 5000, Australia.

Molecular and Cellular Biology, April 2007, p. 2870-2885, Vol. 27, No. 8
0270-7306/07/$08.00+0     doi:10.1128/MCB.02323-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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