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Molecular and Cellular Biology, October 2008, p. 6439-6451, Vol. 28, No. 20
0270-7306/08/$08.00+0     doi:10.1128/MCB.00727-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

An Allelic Series Uncovers Novel Roles of the BRCT Domain-Containing Protein PTIP in Mouse Embryonic Vascular Development{triangledown}

Weipeng Mu, Wei Wang, and John C. Schimenti*

Department of Biomedical Sciences, Cornell College of Veterinary Medicine, Ithaca, New York 14853

Received 7 May 2008/ Returned for modification 11 June 2008/ Accepted 9 August 2008

Pax transactivation domain-interacting protein (PTIP, or PAXIP1) is required for mouse development and has been implicated in DNA damage responses and histone modification. However, the physiological roles of PTIP during embryogenesis remain unclear due to early embryonic lethality of null mutants. We describe two N-ethyl N-nitrosourea-induced hypomorphic missense alleles of Ptip, each of which alters one of the six encoded BRCT domains. Phenotypic characterization of these mutants revealed important functions of PTIP in vasculogenesis and chorioplacental development that appear unrelated to activities in DNA repair or global histone modification. The results of gene expression profiling and in vitro angiogenesis assays indicated that PTIP modulates a transcriptional program, centered around Vegfa, that drives the migration of endothelial cells to properly form the embryonic vasculature. These and other data suggest that PTIP has multiple functions, one of which is to promote the formation of transcriptional complexes that provide specificity of developmental gene expression.

* Corresponding author. Mailing address: Department of Biomedical Sciences, Cornell College of Veterinary Medicine, Ithaca, NY 14853. Phone: (607) 253-3636. Fax: (607) 253-3789. E-mail: jcs92{at}cornell.edu

{triangledown} Published ahead of print on 18 August 2008.

Molecular and Cellular Biology, October 2008, p. 6439-6451, Vol. 28, No. 20
0270-7306/08/$08.00+0     doi:10.1128/MCB.00727-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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