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Molecular and Cellular Biology, December 2008, p. 7414-7426, Vol. 28, No. 24
0270-7306/08/$08.00+0     doi:10.1128/MCB.01237-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation{triangledown} ,{dagger}

Yuxin Zhai, Zhenping Zhong, Chyi-Ying A. Chen, Zhenfang Xia, Ling Song, Michael R. Blackburn, and Ann-Bin Shyu*

Department of Biochemistry and Molecular Biology, The University of Texas Medical School at Houston, Houston, Texas 77030

Received 5 August 2008/ Returned for modification 8 September 2008/ Accepted 3 October 2008

Bronchial epithelial cells play a pivotal role in airway inflammation, but little is known about posttranscriptional regulation of mediator gene expression during the inflammatory response in these cells. Here, we show that activation of human bronchial epithelial BEAS-2B cells by proinflammatory cytokines interleukin-4 (IL-4) and tumor necrosis factor alpha (TNF-{alpha}) leads to an increase in the mRNA stability of the key chemokines monocyte chemotactic protein 1 and IL-8, an elevation of the global translation rate, an increase in the levels of several proteins critical for translation, and a reduction of microRNA-mediated translational repression. Moreover, using the BEAS-2B cell system and a mouse model, we found that RNA processing bodies (P bodies), cytoplasmic domains linked to storage and/or degradation of translationally silenced mRNAs, are significantly reduced in activated bronchial epithelial cells, suggesting a physiological role for P bodies in airway inflammation. Our study reveals an orchestrated change among posttranscriptional mechanisms, which help sustain high levels of inflammatory mediator production in bronchial epithelium during the pathogenesis of inflammatory airway diseases.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, The University of Texas-Houston Medical School, Houston, TX 77030. Phone: (713) 500-6068. Fax: (713) 500-0652. E-mail: Ann-Bin.Shyu{at}uth.tmc.edu

{triangledown} Published ahead of print on 20 October 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, December 2008, p. 7414-7426, Vol. 28, No. 24
0270-7306/08/$08.00+0     doi:10.1128/MCB.01237-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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