This Article Full Text Full Text (PDF) Supplemental material Other Versions of this Article: MCB.01683-07v1 28/6/1875    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Google Scholar Articles by Kabaha, M. M. Articles by Tzfati, Y. PubMed PubMed Citation Articles by Kabaha, M. M. Articles by Tzfati, Y.

Next Article 

Molecular and Cellular Biology, March 2008, p. 1875-1882, Vol. 28, No. 6
0270-7306/08/$08.00+0     doi:10.1128/MCB.01683-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The 5' Arm of Kluyveromyces lactis Telomerase RNA Is Critical for Telomerase Function ^,

Department of Genetics, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel

Received 12 September 2007/ Returned for modification 3 October 2007/ Accepted 29 December 2007

Telomerase is a ribonucleoprotein reverse transcriptase that copies a short template within its integral telomerase RNA moiety (TER) onto eukaryotic chromosome ends, thus compensating for incomplete replication and degradation. The highly divergent yeast TER is structured in three long arms, with a catalytic core at its center. A binding site for the protein Ku80 is conserved within the 5' arm of TER in Saccharomyces but not in Kluyveromyces budding yeast species. Consistently, KU80 deletion in Kluyveromyces lactis does not affect telomere length, while it causes telomere shortening in Saccharomyces cerevisiae. We found elements in the 5' arm of K. lactis TER that are crucial for telomerase activity and stability. However, we found no indication of the association of Ku80 with this arm. Although the overexpression of Ku80 rescues a particular mutation in K. lactis TER1 that phenocopies a telomerase null mutation, this effect is indirect, caused by the repression of the recombination pathway competing for telomere maintenance. Interestingly, the overexpression of Est3, an essential telomerase protein whose function is still unknown, suppresses the phenotypes of mutations in this arm. These results indicate that the 5' arm of K. lactis TER has critical roles in telomerase function, which may be linked to the function of Est3.

Published ahead of print on 14 January 2008.

Supplemental material for this article may be found at http://mcb.asm.org/.