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Molecular and Cellular Biology, April 2008, p. 2324-2331, Vol. 28, No. 7
0270-7306/08/$08.00+0     doi:10.1128/MCB.01252-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Generation and Characterization of rgs5 Mutant Mice{triangledown}

Maya H. Nisancioglu,1 William M. Mahoney Jr.,3 Dara D. Kimmel,3 Stephen M. Schwartz,3 Christer Betsholtz,1,2* and Guillem Genové1

Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden,1 Department of Medicine, Karolinska Institutet, Stockholm, Sweden,2 Department of Pathology, University of Washington, Seattle, Washington3

Received 12 July 2007/ Returned for modification 17 August 2007/ Accepted 19 December 2007

Regulators of G-protein signaling (RGS) are involved in a wide variety of functions, including olfaction, vision, and cell migration. RGS5 has a perivascular expression pattern and was recently identified as a marker for brain pericytes. This suggests a role for RGS5 in vascular development and pericyte biology. We have created a mouse line which lacks the rgs5 gene and replaced it with a green fluorescent protein (GFP) reporter (rgs5GFP/GFP). The mice are viable and fertile and display no obvious developmental defects, and the vasculature appears to develop normally with proper pericyte coverage. Also, no differences were observed in the vasculature under pathological conditions, such as tumor growth and oxygen-induced retinopathy. The GFP expression in pericytes of rgs5GFP mice allows detection and sorting of these cells, thereby providing a valuable novel tool for pericyte research.

* Corresponding author. Mailing address: Karolinska Institutet, Laboratory of Vascular Biology, Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Scheelesväg 2, Plan4:A3, SE-171 77 Stockholm, Sweden. Phone: 46 8 524 87 960. Fax: 46 8 31 34 45. E-mail: christer.betsholtz{at}ki.se

{triangledown} Published ahead of print on 22 January 2008.

Molecular and Cellular Biology, April 2008, p. 2324-2331, Vol. 28, No. 7
0270-7306/08/$08.00+0     doi:10.1128/MCB.01252-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gaengel, K., Genove, G., Armulik, A., Betsholtz, C. (2009). Endothelial-Mural Cell Signaling in Vascular Development and Angiogenesis. Arterioscler. Thromb. Vasc. Bio. 29: 630-638 [Abstract] [Full Text]  
  • Manzur, M., Hamzah, J., Ganss, R. (2009). Modulation of G Protein Signaling Normalizes Tumor Vessels. Cancer Res. 69: 396-399 [Abstract] [Full Text]  


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