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Molecular and Cellular Biology, January 2009, p. 93-103, Vol. 29, No. 1
0270-7306/09/$08.00+0     doi:10.1128/MCB.00704-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

p53 Chromatin Epigenetic Domain Organization and p53 Transcription{triangledown} ,{dagger}

Chia-Hsin Su,1 Yih-Jyh Shann,1 and Ming-Ta Hsu1,2,3*

Institute of Biochemistry and Molecular Biology, School of Life Science,1 Genome Research Center, National Yang Ming University, Taipei 11221, Taiwan, Republic of China,2 Chien-Tien Hsu Cancer Research Foundation, Taipei, Taiwan, Republic of China3

Received 30 April 2008/ Returned for modification 25 June 2008/ Accepted 3 October 2008

Epigenetic organization represents an important regulation mechanism of gene expression. In this work, we show that the mouse p53 gene is organized into two epigenetic domains. The first domain is fully unmethylated, associated with histone modifications in active genes, and organized in a nucleosome-free conformation that is deficient in H2a/H2b, whereas the second domain is fully methylated, associated with deacetylated histones, and organized in a nucleosomal structure. In mitotic cells, RNA polymerase is depleted in domain II, which is folded into a higher-order structure and is associated with H1 histone, whereas domain I conformation is preserved. Similar results were obtained for cells treated with inhibitors of associated regulatory factors. These results suggest that depletion of RNA polymerase II is the result of a physical barrier due to the folding of chromatin in domain II. The novel chromatin structure in the first domain during mitosis also suggests a mechanism for marking active genes in successive cell cycles.

* Corresponding author. Mailing address: National Yang-Ming University No. 155, Sec. 2, Linong Street, Taipei 112, Taiwan. Phone: 886-2-28267230. Fax: 886-2-28264843. E-mail: mth{at}ym.edu.tw

{triangledown} Published ahead of print on 20 October 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, January 2009, p. 93-103, Vol. 29, No. 1
0270-7306/09/$08.00+0     doi:10.1128/MCB.00704-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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