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Molecular and Cellular Biology, July 2009, p. 3644-3656, Vol. 29, No. 13
0270-7306/09/$08.00+0     doi:10.1128/MCB.00851-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Dynamin Reduces Pyk2 Y402 Phosphorylation and Src Binding in Osteoclasts {triangledown} ,{dagger}

Angela Bruzzaniti,1,2* Lynn Neff,2,3 Amanda Sandoval,2 Liping Du,1 William C. Horne,2,3 and Roland Baron2,3,4*

Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, Indiana 46202,1 Department of Orthopedics, Yale University School of Medicine, New Haven, Connecticut 06510,2 Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts 02115,3 Department of Medicine, Harvard Medical School, Boston, Massachusetts 021154

Received 28 May 2008/ Returned for modification 18 July 2008/ Accepted 3 April 2009

Signaling via the Pyk2-Src-Cbl complex downstream of integrins contributes to the assembly, organization, and dynamics of podosomes, which are the transient adhesion complexes of highly motile cells such as osteoclasts and dendritic cells. We previously demonstrated that the GTPase dynamin is associated with podosomes, regulates actin flux in podosomes, and promotes bone resorption by osteoclasts. We report here that dynamin associates with Pyk2, independent of dynamin's GTPase activity, and reduces Pyk2 Y402 phosphorylation in a GTPase-dependent manner, leading to decreased Src binding to Pyk2. Overexpressing dynamin decreased the macrophage colony-stimulating factor- and adhesion-induced phosphorylation of Pyk2 in osteoclastlike cells, suggesting that dynamin is likely to regulate Src-Pyk2 binding downstream of integrins and growth factor receptors with important cellular consequences. Furthermore, catalytically active Src promotes dynamin-Pyk2 association, and mutating specific Src-phosphorylated tyrosine residues in dynamin blunts the dynamin-induced decrease in Pyk2 phosphorylation. Thus, since Src binds to Pyk2 through its interaction with phospho-Y402, our results suggest that Src activates a negative-feedback loop downstream of integrin engagement and other stimuli by promoting both the binding of dynamin to Pyk2-containing complexes and the dynamin-dependent decrease in Pyk2 Y402 phosphorylation, ultimately leading to the dissociation of Src from Pyk2.

* Corresponding author. Mailing address for Angela Bruzzaniti: Department of Oral Biology, Indiana University School of Dentistry, 1121 W. Michigan St., DS241, Indianapolis, IN 46202-5186. Phone: (317) 278-3742. Fax: (317) 278-1411. E-mail: abruzzan{at}iupui.edu. Mailing address for Roland Baron: Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, 188 Longwood Ave., REB309, Boston, MA 02115. Phone: (617) 432-7320. Fax: (617) 432-1879. E-mail: roland_baron{at}hsdm.harvard.edu

{triangledown} Published ahead of print on 20 April 2009.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, July 2009, p. 3644-3656, Vol. 29, No. 13
0270-7306/09/$08.00+0     doi:10.1128/MCB.00851-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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