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Molecular and Cellular Biology, March 2009, p. 1389-1400, Vol. 29, No. 6
0270-7306/09/$08.00+0     doi:10.1128/MCB.01346-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

TFIIB Recognition Elements Control the TFIIA-NC2 Axis in Transcriptional Regulation{triangledown} ,{dagger}

Wensheng Deng,1 Barbora Malecová,2 Thomas Oelgeschläger,2 and Stefan G. E. Roberts1*

Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT,1 Transcription Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom2

Received 24 August 2008/ Returned for modification 22 October 2008/ Accepted 20 December 2008

TFIIB recognizes DNA sequence-specific motifs that can flank the TATA elements of the promoters of protein-encoding genes. The TFIIB recognition elements (BREu and BREd) can have positive or negative effects on transcription in a promoter context-dependent manner. Here we show that the BREs direct the selective recruitment of TFIIA and NC2 to the promoter. We find that TFIIA preferentially associates with BRE-containing promoters while NC2 is recruited to promoters that lack consensus BREs. The functional relevance of the BRE-dependent recruitment of TFIIA and NC2 was determined by small interfering RNA-mediated knockdown of TFIIA and NC2, both of which elicited BRE-dependent effects on transcription. Our results confirm the established functional reciprocity of TFIIA and NC2. However, our findings show that TFIIA assembly at BRE-containing promoters results in reduced transcriptional activity, while NC2 acts as a positive factor at promoters that lack functional BREs. Taken together, our results provide a basis for the selective recruitment of TFIIA and NC2 to the promoter and give new insights into the functional relationship between core promoter elements and general transcription factor activity.

* Corresponding author. Mailing address: Faculty of Life Sciences, Michael Smith Building, University of Manchester, Oxford Road, Manchester M13 9PT, United Kingdom. Phone: 0161 275 5758. Fax: 0161 275 5082. E-mail: stefan.roberts{at}manchester.ac.uk

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://mcb.asm.org/.

Molecular and Cellular Biology, March 2009, p. 1389-1400, Vol. 29, No. 6
0270-7306/09/$08.00+0     doi:10.1128/MCB.01346-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bonham, A. J., Neumann, T., Tirrell, M., Reich, N. O. (2009). Tracking transcription factor complexes on DNA using total internal reflectance fluorescence protein binding microarrays. Nucleic Acids Res 37: e94-e94 [Abstract] [Full Text]  


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