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Mol Cell Biol. 1985 April; 5(4): 898-901

Drosophila P element-enhanced transfection in mammalian cells.

D W Clough, H M Lepinske, R L Davidson and R V Storti

ABSTRACT

We constructed a gene transfer vector containing the herpes simplex virus type 1 thymidine kinase (TK) gene flanked by Drosophila P element terminal repeats (W. R. Engels, Annu. Rev. Genet. 17:315-344). This vector was introduced into mouse LTK- cells and enhanced the frequency of stable transformation to the TK+ phenotype by approximately 50-fold relative to a similar plasmid lacking the P element terminal repeats.

Mol Cell Biol. 1985 April; 5(4): 898-901






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